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公司介绍
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等
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Lucigen/Expresso® Solubility and Expression Screening System/49060-1/24 rxns (3 rxns for each vector)

Lucigen/Expresso® Solubility and Expression Screening System/49060-1/24 rxns (3 rxns for each vector)
  • Lucigen/Expresso® Solubility and Expression Screening System/49060-1/24 rxns (3 rxns for each vector)
商品介绍

Multiplexyourproteinsolubilityscreen

Oneconvenientscreeningkitcontaining7fusiontagsproventoincrease
proteinexpressionandsolubility

  • Useoneamplicontosimultaneouslygenerateallyourconstructs
  • Rapid,simultaneousproteinscreeningwithTEV-cleavablefusiontags
  • PCRtoproteininaslittleas4days

  • Overview
  • Simplifiedworkflow
  • Results
  • Fusiontagsincluded
  • ProteincleavagewithSelecTEV™Protease
  • FAQ's

Overview

TheExpresso®SolubilityandExpressionScreeningSystemisanall-in-onesystemthatenablesPCRamplification,rapidcloning,andexpressionofyourproteinofinterestwithadiversepanelofpowerfulandcleavablesolubilitytags.Lucigenhasidentified7uniqueandliterature-validatedsolubilitytagsandcombinedthemwithExpressioneering™technologytocreateasuiteofcloning-readyvectorsforrapidandparallelevaluationofyourproteinwithall7solubilitytags(plusa6xHis-taggedcontrolvector).

pSOL Vector

Figure1.EachpSOLvectorcontainseverythingyouneedtoseamlesslycloneinyourtargetgenewithoutanyadditionalaminoacidsadded. FusiontagsarecleavableviaaTEVproteasesite. 

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SimplifiedWorkflow

Expresso Screening Workflow

Lucigen'sExpressioneering™technologymakesitsimplerthanevertorapidlyscreenmultiplefusiontagsforsolubilityandexpressionenhancement. Useoneamplicontosimultaneouslycreate8differentclones(7fusiontagsandoneHis-taggedcontrol),followedbyscreeningforsolubilityandexpression.

WanttolearnmoreaboutExpressioneering? Watchourvideo

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Results

Everyproteinreactsdifferentlytovariousfusiontags. WiththeExpressoSolubilityandExpressionScreeningSystem,youcanquicklyandeasilyscreenmultiple,provenfusiontagstofindtheoptimaltagforyourprotein.

GH1
Figure2.GH1(HumanGrowthHormone)wasevaluatedforE.coliexpressionandsolubilitywhenexpressedasafusion.FusiontagsSlyD,Tsf,SUMO,Bla,andMBPdemonstratedenhancedsolubility(LaneS)comparedtotheHis-controlprotein.T:TotalLysate,S:ClearedSolubleFraction,I:InsolublePellet

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FusionTagsIncluded

TheExpressoSolubilityandExpressionScreeningSystemincludes7vectorsthateachcontainauniquefusiontagandoneHiscontrolvector.Thefusiontagsvaryinsequence,size,andderivative.Belowisatablesummarizingthecharacteristicsofeachfusiontag:

#

FusionTag

AALength

kDa*

pI

Description

1

6xHis-AFV

113

13.5

5.0

HypotheticalproteinfromAcidianusfilamentousvirus1

2

6xHis-SlyD

210

22.7

5.2

FKBP-typepeptidyl-prolylcis-transisomerase

3

6xHis-Tsf

297

32.2

5.7

E.colielongationfactor

4

6xHis-SUMO

115

13.3

5.2

SmallUbiquitin-likeModifier

5

6xHis-Bla

381

41.3

4.4

Beta-lactamase

6

6xHis-MBP

382

42.1

5.5

Maltose-BindingProtein

7

6xHis-GST

233

27.4

6.6

GlutathioneS-transferase

8

6xHisControl

14

1.8

7.0

Affinitytag

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CleavagewithSelecTEV®Protease

AllfusiontaggedproteinsproducedwiththeExpressoSolubilityandExpressionScreeningSystemarefullycleavablewithSelecTEVproteasefromLucigen. Formoreinformation,visittheSelecTEVproteaseproductpage

SelecTEV Protease
Figure3.Coomassie-stainedSDS-PAGEgelofTEVcleavagereaction.10UofSelecTEVProteasewasincubatedwith30µgoffusionproteinsubstrateat30°Cfor1hourina100µLreaction.Marker(laneM),SelecTEVProtease(Lane1),Uncleavedfusionsubstrate(Lane2),CleavedsubstrateandSelecTEVProtease.(Lane3)

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ORDERINFORMATION

TheExpressoSolubilityandExpressionScreeningSystemcontainspre-processedpSol™vectorswith7differentfusiontagsandoneHIS-taggedcontrolvector(3reactionsofeachvector),positivecontrolinsert,sequencingprimers,E.cloni®10GChemicallyCompetentCells(SOLOs),and20%Rhamnosesolutionand15%Glucosesolutionautoinductionreagents.SelecTEV™ProteaseandAccura®High-FidelityPolymeraseareavailableforpurchaseseparately.

Forresearchuseonly.Completeprotocolsareavailableonlineatwww.lucigen.com/manuals.

品牌介绍

美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。


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美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。

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