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公司介绍
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,...
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Lucigen/Expresso® Solubility and Expression Screening System + SelecTEV™ Protease + Accura® High-Fidelity Polymeras

  • 产品编号: 49066-1
  • 美  元  价: $1080.00
  • 会  员  价: 待定
  • 品       牌: Lucigen
  • 产       地: 美国
  • 公       司: Lucigen
  • 产品分类: ref="." class="index">首页 > Lucigen/Expresso® Solubility and Expression Screening System + SelecTEV™ Protease + Accura® High-Fidelity Polymeras
  • 公司分类: Bacterial Expression
:24 rxns + 1,000 Units SelecTEV + 100 units Accura
Lucigen/Expresso® Solubility and Expression Screening System + SelecTEV™ Protease + Accura® High-Fidelity Polymeras
  • Lucigen/Expresso® Solubility and Expression Screening System + SelecTEV™ Protease + Accura® High-Fidelity Polymeras
商品介绍

Multiplex your protein solubility screen

One convenient screening kit containing 7 fusion tags proven to increase
protein expression and solubility

  • Use one amplicon to simultaneously generate all your constructs
  • Rapid, simultaneous protein screening with TEV-cleavable fusion tags
  • PCR to protein in as little as 4 days

  • Overview
  • Simplified workflow
  • Results
  • Fusion tags included
  • Protein cleavage with SelecTEV™ Protease
  • FAQ's

Overview

The Expresso® Solubility and Expression Screening System is an all-in-one system that enables PCR amplification, rapid cloning, and expression of your protein of interest with a diverse panel of powerful and cleavable solubility tags. Lucigen has identified 7 unique and literature-validated solubility tags and combined them with Expr essioneering™ technology to create a suite of cloning-ready vectors for rapid and parallel evaluation of your protein with all 7 solubility tags (plus a 6xHis-tagged control vector).

pSOL Vector

Figure 1. Each pSOL vector contains everything you need to seamlessly clone in your target gene without any additional amino acids added.  Fusion tags are cleavable via a TEV protease site. 

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Simplified Workflow

Expresso Screening Workflow

Lucigen's Expressioneering™ technology makes it simpler than ever to rapidly screen multiple fusion tags for solubility and expression enhancement.  Use one amplicon to simultaneously create 8 different clones (7 fusion tags and one His-tagged control), followed by screening for solubility and expression.

Want to learn more about Expressioneering?  Watch our video

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Results

Every protein reacts differently to various fusion tags.  With the Expresso Solubility and Expression Screening System, you can quickly and easily screen multiple, proven fusion tags to find the optimal tag for your protein.

GH1
Figure 2. GH1 (Human Growth Hormone) was evaluated for E. coli expression and solubility when expressed as a fusion. Fusion tags SlyD, Tsf, SUMO, Bla, and MBP demonstrated enhanced solubility (Lane S) compared to the His-control protein. T: Total Lysate, S: Cleared Soluble Fraction, I: Insoluble Pellet

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Fusion Tags Included

The Expresso Solubility and Expression Screening System includes 7 vectors that each contain a unique fusion tag and one His control vector. The fusion tags vary in sequence, size, and derivative. Below is a table summarizing the characteristics of each fusion tag:

#

Fusion Tag

AA Length

kDa*

pI

Description

1

6xHis-AFV

113

13.5

5.0

Hypothetical protein from Acidianus filamentous virus 1

2

6xHis-SlyD

210

22.7

5.2

FKBP-type peptidyl-prolyl cis-trans isomerase

3

6xHis-Tsf

297

32.2

5.7

E. coli elongation factor

4

6xHis-SUMO

115

13.3

5.2

Small Ubiquitin-like Modifier

5

6xHis-Bla

381

41.3

4.4

Beta-lactamase

6

6xHis-MBP

382

42.1

5.5

Maltose-Binding Protein

7

6xHis-GST

233

27.4

6.6

Glutathione S-transferase

8

6xHis Control

14

1.8

7.0

Affinity tag

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Cleavage with SelecTEV® Protease

All fusion tagged proteins produced with the Expresso Solubility and Expression Screening System are fully cleavable with SelecTEV protease from Lucigen.  For more information, visit the SelecTEV protease product page

SelecTEV Protease
Figure 3. Coomassie-stained SDS-PAGE gel of TEV cleavage reaction. 10 U of SelecTEV Protease was incubated with 30 µg of fusion protein substrate at 30°C for 1 hour in a 100 µL reaction. Marker (lane M), SelecTEV Protease (Lane 1), Uncleaved fusion substrate (Lane 2), Cleaved substrate and SelecTEV Protease. (Lane 3)

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ORDER INFORMATION

The Expresso Solubility and Expression Screening System contains pre-processed pSol™ vectors with 7 different fusion tags and one HIS-tagged control vector (3 reactions of each vector), positive control insert, sequencing primers, E. cloni® 10G Chemically Competent Cells (SOLOs), and 20% Rhamnose solution and 15% Glucose solution autoinduction reagents. SelecTEV™ Protease and Accura® High-Fidelity Polymerase are available for purchase separately.

For research use only. Complete protocols are available online at www.Lucigen.com/manuals.

品牌介绍

美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。

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