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公司介绍
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等
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Lucigen/TargetAmp™-Nano Labeling Kit for Illumina Expression BeadChip/TAN091096/96 Reactions

Lucigen/TargetAmp™-Nano Labeling Kit for Illumina Expression BeadChip/TAN091096/96 Reactions
  • Lucigen/TargetAmp™-Nano Labeling Kit for Illumina Expression BeadChip/TAN091096/96 Reactions
商品介绍

Optimizedforhighsignalintensity

  • Formerly from EpicentreFast.Performthe6-hourreactionandbeginBeadChiphybridizationthesameday.
  • HighSensitivity.SensitiveandreproducIBLegenedetection.
  • HighYield.Makemicrogramamountsofbiotin-aRNA(biotin-cRNA)fromaslittleas25ngoftotalRNA.
  • HighFidelity.TargetAmp-Nanolabelingprocedurepreservestherelativeabundanceoftheoriginalsample.

TheTargetAmp™-NanoLabelingKitproducesbiotin-aRNA(alsocalledbiotin-cRNA)thathasbeenoptimizedforhighsignalintensityonIlluminaExpressionBeadChiparrays,enablingsensitivegenedetectionandreproducibledifferentialexpressionresults.

Benefits

  • Fast.Performthe6-hourreactionandbeginBeadChiphybridizationthesameday.
  • HighSensitivity.Sensitiveandreproduciblegenedetection.
  • HighYield.Makemicrogramamountsofbiotin-aRNA(biotin-cRNA)fromaslittleas25ngoftotalRNA.
  • HighFidelity.TargetAmp-Nanolabelingprocedurepreservestherelativeabundanceoftheoriginalsample.

Table1.TargetAmp-NanoKitaGuide

InputRNAReaction
Time
Additional
TargetAmp-NanoKit25ng-500ngtotalRNA6hoursRequiresuseofSuperScriptIIIReverseTranscriptase
(providedbytheuser)

Table2.YieldofBiotin-aRNAfromTargetAmp-NanoKit

InputRNA(HeLatotalRNA)50pg100pg500pg25ng100ng500ng
TargetAmp-NanoKitreaction3μg10μg75μg

ORDERINFORMATION

ThesekitswereoptimizedusingSuperScript™IIIreversetranscriptase(Invitrogen),whichisnotincludedinthekits.Contents:TargetAmp™T7-Oligo(dT)Primer,TargetAmp™Nano1st-StrandCDNAPremix,TargetAmp™Nano2nd-StrandcDNAPremix,TargetAmp™Nano2nd-StrandcDNADNAPolymerase,TargetAmp™NanoT7RNAPolymerase,TargetAmp™NanoT7TranscriptionBuffer,TargetAmp™NanoNTPPreMix,TargetAmp™NanoUTP/Biotin-UTP,RNase-FreeDNaseI,DTT,HeLaTotalRNAControl,RNase-FreeWater.
品牌介绍

美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。

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