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Lucigen/MaxPlax™ Lambda Packaging Extracts/MP5105/5 Extracts
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> Lucigen/MaxPlax™ Lambda Packaging Extracts/MP5105/5 Extracts| Size: | 5 Extracts |
Construct genomic libraries from methylated or unmethylated DNA with equal efficiency due to the lack of all known restriction activities in the extracts.MaxPlax™ LamBDa Packaging Extracts offer maximum in vitro packaging efficiencies of cos site-containing methylated or unmethylated DNA. TrADItional lamBDa packaging extracts are derived from two complementary lysogenic E. coli strains, BHB2690 and BHB2688, as described by Hohn.1 MaxPlax Extracts use a restriction-free E. coli K-12 strain, NM759, in place of strain BHB2690.2 The sonicated extracts of NM759 are combined with extracts of BHB2688 to produce MaxPlax Extracts.
Each lot of MaxPlax Extracts is tested and guaranteed to maintain a packaging efficiency of greater than 1 × 109 pfu/µg of Control Ligated LamBDa DNA for 1 year from the date of purchase if stored properly. A Certificate of Analysis stating actual packaging efficiency (pfu/µg DNA) is provided with each lot of MaxPlax Extracts.
| Table 1. Packaging of methylated and unmethylated DNA using MaxPlax™ LamBDa Packaging Extracts. | |
| Cosmid Insert | Pfu/µg DNA |
| Unmethylated DNA | 3.5 x 107 |
| Methylated DNA | 3.9 x 107 |
References
美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。
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