请使用支持JavaScript的浏览器! Lucigen-Difficult Cloning or BAC/Fosmid Cloning-Lucigen/MaxPlax™ Lambda Packaging Extracts/MP5105/5 Extracts
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公司介绍
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,...
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Lucigen/MaxPlax™ Lambda Packaging Extracts/MP5105/5 Extracts

  • 产品编号: MP5105
  • 美  元  价: $188.00
  • 会  员  价: 待定
  • 品       牌: Lucigen
  • 产       地: 美国
  • 公       司: Lucigen
  • 产品分类: ref="." class="index">首页 > Lucigen/MaxPlax™ Lambda Packaging Extracts/MP5105/5 Extracts
  • 公司分类: Difficult Cloning or BAC/Fosmid Cloning
Size:5 Extracts
Lucigen/MaxPlax™ Lambda Packaging Extracts/MP5105/5 Extracts
  • Lucigen/MaxPlax™ Lambda Packaging Extracts/MP5105/5 Extracts
商品介绍

Maximized packaging efficiency of lamBDa, cosmid, or fosmid DNA for the construction of large insert genomic libraries.

  • Formerly from EpicentreConstruct genomic libraries from methylated or unmethylated DNA with equal efficiency due to the lack of all known restriction activities in the extracts.
  • Packaging efficiencies of up to 3 × 109 pfu/µg DNA.
  • Simplified protocol does not require premixing of different extract components before use.

MaxPlax™ LamBDa Packaging Extracts offer maximum in vitro packaging efficiencies of cos site-containing methylated or unmethylated DNA. TrADItional lamBDa packaging extracts are derived from two complementary lysogenic E. coli strains, BHB2690 and BHB2688, as described by Hohn.1 MaxPlax Extracts use a restriction-free E. coli K-12 strain, NM759, in place of strain BHB2690.2 The sonicated extracts of NM759 are combined with extracts of BHB2688 to produce MaxPlax Extracts.

Each lot of MaxPlax Extracts is tested and guaranteed to maintain a packaging efficiency of greater than 1 × 109 pfu/µg of Control Ligated LamBDa DNA for 1 year from the date of purchase if stored properly. A Certificate of Analysis stating actual packaging efficiency (pfu/µg DNA) is provided with each lot of MaxPlax Extracts.

Table 1. Packaging of methylated and unmethylated DNA using MaxPlax™ LamBDa Packaging Extracts.
Cosmid Insert Pfu/µg DNA
Unmethylated DNA 3.5 x 107
Methylated DNA 3.9 x 107

References

  1. Hohn, E.G. (1979) Methods Enzymol. 68, 299.
  2. Gunther, E.G. et al. (1993) Nucleic Acids Res. 21, 3903.
  3. Fiandt, M. (2000) Epicentre Forum 7(3), 14.

ORDER INFORMATION

Contents: Predispensed MaxPlax™ Extracts, Control LamBDa DNA, Control Host Cells (E. coli).
品牌介绍

美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。

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