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主营:体外转录、基因克隆(BAC克隆试剂盒)、感受态细胞(TG1噬菌体文库构建感受态)、体外转录、转座子突变、蛋白表达
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当前位置: 首页 > 产品中心 > Difficult Cloning or BAC/Fosmid Cloning > Lucigen/CopyRight v2.0 BAC Cloning Kit, pSMART BAC HindIII w/BAC-Optimized Replicator v2.0 Electrocompetent Cells/42032-
公司介绍
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等
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Lucigen/CopyRight v2.0 BAC Cloning Kit, pSMART BAC HindIII w/BAC-Optimized Replicator v2.0 Electrocompetent Cells/42032-

Lucigen/CopyRight v2.0 BAC Cloning Kit, pSMART BAC HindIII w/BAC-Optimized Replicator v2.0 Electrocompetent Cells/42032-
  • Lucigen/CopyRight v2.0 BAC Cloning Kit, pSMART BAC HindIII w/BAC-Optimized Replicator v2.0 Electrocompetent Cells/42032-
商品介绍
  • Efficientlycloneanysizeinsertupto200kb.
  • CreatelibrariesfromA/T-orG/C-richgenomes.
  • Clonegeneclustersoroperons.
  • HighinsertstABIlity.
  • InducIBLecopynumber.

Lucigen'sCopyRight®v2.0BACcloningkitswithpSMART®BAC(Figure1)orpEZBAC(Figure2)vectorandBAC-OptimizedReplicator™v2.0ElectrocompetentCellsresultinunprecedentedaccuracy,reliability,andefficiencyinBACcloningandlibraryconstruction.ThesevectorsincorporatetheCloneSmart®transcription-freetechnology,whichminimizescloningbiasandenablescloningofotherwisetoxicgenes,alongwithinduciblecopynumberamplification(20-50X)forhighDNAyieldsandalacZ-sacBstufferfragmentthateliminatesuncutvectorfromrecombinants,resultinginzerobackground. UnlikeotherBACcloningsystems,Lucigen'sCloneSmarttechnologyassuresthateven"unclonable"insertsarestablymaintained.Sequencesarenotlostorrearrangedinthecloningprocess. CopyRightKitsoffersuperiorperformanceforBACcloningatamuchbetterpricethanotherkits. Figure3showsanexampleofaRandomShearBAClibrarygeneratedwithpSMARTBAC.

pSMART BAC Vector Map

pEZ BAC Vector Map

Figure1.CopyRightvectorpSMARTBAC. ori2,repE,IncC-single-copyoriginofreplication;oriV-inducibleoriginofreplication;parA,B,C-partitiongenes;Cm^r-chloramphenicol-resistancegene;cosN-lamBDapackagingsignal;T-CloneSmarttranscriptionterminators;sacB-sucrasegene.Approximatepositionsofsequencingprimersandtranscriptionterminatorsareindicated.

Figure2.CopyRightvectorpEZBAC.ori2,repE,IncC,single-copyoriginofreplication;oriV-inducibleoriginofreplication;parA,B,C,partitiongenes;Cm^r-chloramphenicol-resistancegene;cosN-lambdapackagingsignal;lacZ-alphapeptideofthebetagalactosidasegene.Approximatepositionsofsequencingprimersandtranscriptionterminatorsareindicated.

 

Arabidopsis BAC gel

Figure3.ArabidopsisRandomShearBACLibraryinthepSMARTBACvector.7,680cloneswereobtained,withanaverageinsertsizeof105kband~5Xgenomecoverage.

ConvenientSuccess
Efficientlyobtainyourcloneorcreateyourlibrary—theCopyRightv2.0CloningKitseliminatetediousvectorandcompetentcellpreparation,aswellastime-consumingQCexperiments. Kitsincludeoptimizedreagents,ligation-readyvector,highlyefficientelectrocompetentcells,detailedinstructions,andatrouble-shootingguide.

ORDERINFORMATION

EachCopyRight®v2.0BACCloningKitcontains:pre-cutvector(pSMARTBACwithachoiceofBamHIorHindIIIsites),CopyRightDNALigase,CopyRight5XLigationBuffer,PositiveControlInsertDNA,SequencingPrimers,BAC-OptimizedReplicatorv2.0ElectrocompetentCellsinDUOpackaging(2transformationspertube),PositiveControlInsertDNA,RecoveryMedium,ArabinoseInductionSolution,andcompleteprotocols.EachCopyRightpEZBACCloningKitcontains:pre-cutCopyRightvector(pEZBACBlunt),CloneSmartDNALigase,CloneDirect™10XLigationBuffer(includesATP),PositiveControlInsertDNA,ReplicatorInductionSolution,SequencingPrimers,andcompleteprotocols.KitswithcellsalsoincludeBAC-OptimizedReplicatorv2.0ElectrocompetentCells(>1×1010 cfu/µgDNA)packagedintwotransformationspertube(DUOs),PositiveControlPlasmid,andRecoveryMedium.

Pleasenotethatthefollowingvectorshavebeendiscontinued:pSMARTBACEcoRI(catalognumbers42031-1,42031-2)andpEZBACBamHI(catalognumbers42007-1,42007-2and42012-1).”

品牌介绍

美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。

Lucigen有各种各样的克隆系统,包括新型的无转录克隆载体,可提高克隆产量,降低克隆偏倚并提高测序通量。为了克隆最困难的序列,BigEasy®v2.0线性克隆系统提供了无与伦比的稳定性。大多数Lucigen克隆载体都是预先切割,去磷酸化的,可以立即使用。

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