请使用支持JavaScript的浏览器! Lucigen-Difficult Cloning or BAC/Fosmid Cloning-Lucigen/CopyRight v2.0 pEZ BAC Blunt Cloning Kit w/Electrocompetent Cells/42009-2/20 rxns
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公司介绍
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,...
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Lucigen/CopyRight v2.0 pEZ BAC Blunt Cloning Kit w/Electrocompetent Cells/42009-2/20 rxns

  • 产品编号: 42009-2
  • 美  元  价: $945.00
  • 会  员  价: 待定
  • 品       牌: Lucigen
  • 产       地: 美国
  • 公       司: Lucigen
  • 产品分类: ref="." class="index">首页 > Lucigen/CopyRight v2.0 pEZ BAC Blunt Cloning Kit w/Electrocompetent Cells/42009-2/20 rxns
  • 公司分类: Difficult Cloning or BAC/Fosmid Cloning
Size:20 rxns
Lucigen/CopyRight v2.0 pEZ BAC Blunt Cloning Kit w/Electrocompetent Cells/42009-2/20 rxns
  • Lucigen/CopyRight v2.0 pEZ BAC Blunt Cloning Kit w/Electrocompetent Cells/42009-2/20 rxns
商品介绍
  • Efficiently clone any size insert up to 200kb.
  • Create libraries from A/T- or G/C-rich genomes.
  • Clone gene clusters or operons.
  • High insert stABIlity.
  • InducIBLe copy number.

Lucigen's CopyRight® v2.0 BAC cloning kits with pSMART® BAC (Figure1) or pEZ BAC (Figure 2) vector and BAC-Optimized Replicator™ v2.0 Electrocompetent Cells result in unprecedented accuracy, reliABIlity, and efficiency in BAC cloning and library construction. These vectors incorporate the CloneSmart® transcription-free technology, which minimizes cloning bias and enables cloning of otherwise toxic genes, along with inducIBLe copy number amplification (20-50X) for high DNA yields and a lacZ-sacB stuffer fragment that eliminates uncut vector from recombinants, resulting in zero background.  Unlike other BAC cloning systems, Lucigen's CloneSmart technology assures that even "unclonable" inserts are stably maintained. Sequences are not lost or rearranged in the cloning process.  CopyRight Kits offer superior performance for BAC cloning at a much better price than other kits.  Figure 3 shows an example of a Random Shear BAC library generated with pSMART BAC.

pSMART BAC Vector Map

pEZ BAC Vector Map

Figure 1. CopyRight vector pSMART BAC.  ori2, repE, IncC - single-copy origin of replication; oriV - inducIBLe origin of replication; par A,B,C - partition genes; Cm^r - chloramphenicol-resistance gene; cosN - lamBDa packaging signal; T - CloneSmart transcription terminators; sacB -sucrase gene. Approximate positions of sequencing primers and transcription terminators are indicated.

Figure 2. CopyRight vector pEZ BAC. ori2, repE, IncC, single-copy origin of replication; oriV - inducIBLe origin of replication; par A,B,C, partition genes; Cm^r - chloramphenicol-resistance gene; cosN - lamBDa packaging signal; lacZ - alpha peptide of the beta galactosidase gene. Approximate positions of sequencing primers and transcription terminators are indicated.

 

Arabidopsis BAC gel

Figure 3. ArABIdopsis Random Shear BAC Library in the pSMART BAC vector. 7,680 clones were obtained, with an average insert size of 105 kb and ~5X genome coverage.

Convenient Success
Efficiently obtain your clone or create your library—the CopyRight v2.0 Cloning Kits eliminate tedious vector and competent cell preparation, as well as time-consuming QC experiments.  Kits include optimized reagents, ligation-ready vector, highly efficient electrocompetent cells, detailed instructions, and a trouble-shooting guide.

ORDER INFORMATION

Each CopyRight® v2.0 BAC Cloning Kit contains: pre-cut vector (pSMART BAC with a choice of BamHI or HindIII sites), CopyRight DNA Ligase, CopyRight 5X Ligation Buffer, Positive Control Insert DNA, Sequencing Primers, BAC-Optimized Replicator v2.0 Electrocompetent Cells in DUO packaging (2 transformations per tube), Positive Control Insert DNA, Recovery Medium, ArABInose Induction Solution, and complete protocols. Each CopyRight pEZ BAC Cloning Kit contains: pre-cut CopyRight vector (pEZ BAC Blunt), CloneSmart DNA Ligase, CloneDirect™ 10X Ligation Buffer (includes ATP), Positive Control Insert DNA, Replicator Induction Solution, Sequencing Primers, and complete protocols. Kits with cells also include BAC-Optimized Replicator v2.0 Electrocompetent Cells (>1 × 1010 cfu/µg DNA) packaged in two transformations per tube (DUOs), Positive Control Plasmid, and Recovery Medium.

Please note that the following vectors have been discontinued: pSMART BAC EcoRI (catalog numbers 42031-1, 42031-2) and pEZ BAC BamHI (catalog numbers 42007-1, 42007-2 and 42012-1).”

品牌介绍

美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。

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