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当前位置: 首页 > 产品中心 > General Cloning & Library Construction > Lucigen/E. cloni 10GF ELITE Electrocompetent Cells, > 2 × 10^10 cfu/µg/60061-2/24 rxns (DUOs)
公司介绍
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等
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Lucigen/E. cloni 10GF ELITE Electrocompetent Cells, > 2 × 10^10 cfu/µg/60061-2/24 rxns (DUOs)

Lucigen/E. cloni 10GF ELITE Electrocompetent Cells, > 2 × 10^10 cfu/µg/60061-2/24 rxns (DUOs)
  • Lucigen/E. cloni 10GF ELITE Electrocompetent Cells, > 2 × 10^10 cfu/µg/60061-2/24 rxns (DUOs)
商品介绍
  • Directreplacementsforstandardcloningstrains(e.g.,DH5α™,DH10B™,JM109,TOP10,XL1-Blue,etc.)
  • Optimizedgeneticsforhighyields: phageT1resistant,endonucleaseandrecombinationminus,blue/whitescreening-capable.
  • Availableinarangeofhightransformationefficiencies(5×109to4×1010cfu/µg).
  • Convenientpackagingoptions.

Lookingforchemicallycompetentversion?

Free Sample AvailableFreeSampleAvailable

 

Lucigen’sE.clonicompetentcellssharethemostusefulgeneticelementsofstandardcloningstrainslikeDH5α™,DH10B™,JM109,TOP10,etc.anddirectlyreplacethemincloningprotocols.However,E.clonielectrocompetentcellsincorporateauniquemanufacturingtechnologythatincreasestransformationefficiency,recombinantyieldsandreliABIlity(Figures1and2),whiledecreasingcosts. Thesecellsprovidesolutionsforawiderangeofapplicationsateconomicalprices.

Choiceofstrain:

  • E.cloni10GCompetentCells.Libraryconstruction,cloning,subcloning,andplasmidisolationwithorwithoutblue/whitescreening.
  • E.cloni10GF′CompetentCells.ContaintheF'plasmidforinfectionwithM13toproducessDNA.

Choiceofefficiency:

  • E.cloni10GSUPREMEElectrocompetentCells>4×1010cfu/µgpUCDNA
    SUPREMECellshavethehighesttransformationefficiencyavailablefromanysupplier.ChooseSUPREMECellsforthemostdemandingcloningsituations,suchasconstructionoflarge,highcomplexitylibrariesorcloningdifficulttargets,whichrequirethegreatestnumberoftransformantspossIBLe.
  • E.cloni10G&10GF´ELITEElectrocompetentCells>2×1010cfu/µgpUCDNA
    ELITECellshavetwicethetransformationefficiencycomparedto“ultrahighefficiency”cellsfromothersuppliers.ELITECellsprovidelargenumbersoftransformantsfromhard-to-clonefragmentsorlimitedDNAatalowerprice.
  • E.cloni10GCLASSICElectrocompetentCells>5×109cfu/µgpUCDNA
    CLASSICCellsarehighefficiencycellswithasubstantiallylowercostperreaction.Thesecellsarethemosteconomicalchoiceforstandardcloningandlibraryconstruction.10GCLASSICCellsareavailableinlargerpackagesizesforconvenientuseinhighervolumecloningapplications.

Custom CellsCustomCompetentCells

BulkLucigencellsoryourstrainofE.coli,madetothehighestpossibletransfectionefficiencywithcustomdispensing!SeeCustomCompetentCellspagefordetailedinformation.


Plasmidcloning.E.cloni10GSUPREMECellsproviderecombinantyieldshigherthanthehighestefficiencycellsofferedbyaleADIngsupplier(Figures1and2)

Recombinant Yields

 

Transformation Efficiency

Figure1.E.cloni10GSUPREMEElectrocompetentCellsconsistentlyoutperformed“ultrahighefficiency”cellsfromaleadingsupplier.Bothstrainsweretransformedwith10pgofpUC19(n=16).

 

Figure2.TransformationefficiencycomparisonofE.cloni10GSUPREMECells(Lucigen)andMegaDH10Bcells(Invitrogen).Competentcellsweretransformedaccordingtothemanufacturer’sinstructionswiththesamepUC19DNAcontrol(providedwiththeInvitrogencells)

Table1.E.cloniCompetentCellsforCloningandLibraryConstruction


E.cloniCellLines

TransformationEfficiency
(cfu/µgpUCDNA)

CloningMethylatedDNA

BAC,CosmidCloning

Blue/WhiteScreening

10GSUPREMEElectrocompetent

>4×1010

YES

YES

YES
withoutIPTGinduction

10GELITEElectrocompetent

>2×1010

YES

NO

YES
withoutIPTGinduction

10GCLASSICElectrocompetent

>5×109

YES

NO

YES
withoutIPTGinduction

10GF´ELITEElectrocompetent

>2×1010

YES

NO

YES
IPTGinductionrequired

Efficiencies

  • E.cloni 10GSUPREMEElectrocompetentCells > 4×1010 cfu/µgpUCDNA
  • E.cloni 10G&10GF´ELITEElectrocompetentCells > 2 × 1010 cfu/µgpUCDNA
  • E.cloni 10GCLASSICElectrocompetentCells > 5 × 109 cfu/µgpUCDNA 

ORDERINFORMATION

CompetentCellsincludeControlDNAandRecoveryMedium,andarepackagedasSOLOs(1transformationpertube),DUOs(2transformationspertube),orSixPacks(6transformationspertube).RecoveryMediumisalsoavailableseparately.ThespecifiedtransformationefficienciesarewithpUCDNA,unlessindicatedotherwise.

PLEASENOTE:Bulkquantities(10timeslargerthanthelargestretailpackagesizebelow)and/orcustompackagingareavailableatveryattractivepricesforallLucigenCompetentCells.PleasecontactLucigen.

品牌介绍

美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。


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作为LGC(Biosearch Technologies)的一部分,Lucigen通过定制和OEM生产感受态细胞,高质量酶,核酸提取和纯化试剂,实现了广泛的诊断和研究应用,包括PCR和下一代测序(NGS),和寡核苷酸。为了确保满足您项目的特定需求,我们可以自定义酶,寡核苷酸和提取试剂的浓度,配方,分配,包装和标签-包括私有标签。并且,我们还将从头到尾为您的项目提供知识丰富的科学支持。

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