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当前位置: 首页 > 产品中心 > CRISPR, Large or Difficult Fragment Cloning > Lucigen/TransforMax™ EPI300™ Electrocompetent E. coli/EC300150/50 X 100 µl
公司介绍
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等
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Lucigen/TransforMax™ EPI300™ Electrocompetent E. coli/EC300150/50 X 100 µl

Lucigen/TransforMax™ EPI300™ Electrocompetent E. coli/EC300150/50 X 100 µl
  • Lucigen/TransforMax™ EPI300™ Electrocompetent E. coli/EC300150/50 X 100 µl
商品介绍

StandardcloningforlargeinsertsandGibsonassemblyelectroporation

  • Formerly from EpicentreClonelargeinsertsandtransformlargeplasmids-uptoatleast145kbplasmidDNA.
  • GeneratecloneswithinducIBLecopynumberusingCopyControl™vectors.
  • Achievehightransformationefficiencies:>1×1010cfu/µgpUC19DNA.
  • IncreaseGibsonAssembly®colonycountswithhigh-efficiencyelectroporation
FreeSampleAvailable

Applications

  • Generationofinduciblecopy-numberclonesusingtheCopyControl™CloningSystem.

TransforMax™EPI300™E.colicellslackthetonAgeneandareengineeredforusewithEpicentre'sCopyControl™CDNA,Gene,andPCRCloningKitandotherCopyControlCloningSystems*thatdonotrequirephageT1-resistantcells.ThecellscontainaninduciblemutanttrfAgenewhosegeneproductisrequiredforinitiationofreplicationfromtheoriVoriginofreplication,suchasthatinCopyControlpCC1™vectors.OnLBchloramphenicolplatesorinLBorSOCmediasupplementedwithchloramphenicol,CopyControlclonesgrowninTransforMaxEPI300E.colireplicateatsingle-copynumberfromtheF-factorrepliconbecauseexpressionofthetrfAgeneisrepressed.AdditionofCopyControlInductionSolutioninducesthecellstoexpressthetrfAgeneproductandinducestheirreplicationathigh-copynumberfromoriV(Fig.1).ReplicationfromoriVresultsinhigheryieldsandhigherpurityofclonedDNA.

Benefits

  • Copy-numberofclonesisundertightcontrolofaninduciblepromoterlinkedtothetrfAgene.
  • Hightransformationefficiencywithclonesofallsizes.
  • lacZΔM15forblue/whitescreeningofrecombinants.
  • Restriction-minus[mcrA,Δ(mrr-hsdRMS-mcrBC)]phenotypeenablesefficientcloningofmethylatedDNA.
  • Endonuclease-minus(endA1),toensurehighyieldsofDNA.
  • Recombination-minus(recA1),forgreaterstABIlityoflargeinsertclones.

Figure1.CopynumberofCopyControl™BACclonescanbeinduced10-to20-foldinTransforMax™EPI300™E.coli.TheyieldofBACDNAfromCopyControlBACclonesof110-145kbincreased>14-foldfollowingadditionofCopyControlInductionSolution.U=uninducedcells;I=inducedcells.

Genotype

F-mcrAΔ(mrr-hsdRMS-mcrBC)Φ80dlacZΔM15ΔlacX74recA1endA1araD139Δ(ara,leu)7697galUgalKλ-rpsL(StrR)nupGtrfAdhfr

TransforMaxEPI300ElectrocompetentE.coli

  • Transformationefficiencyof>1x1010cfu/µgofpUC19.

TransforMaxEPI300ChemicallyCompetentE.coli

  • Transformationefficiencyof>5x108cfu/µgofpUC19.

*Coveredbyissuedand/orpendingpatents.


ORDERINFORMATION

1,000Xconcentratedsolution.Filtersterilized.
品牌介绍

美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。

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