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公司介绍
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,...
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Lucigen/TransforMax™ EPI300™ Electrocompetent E. coli/EC300150/50 X 100 µl

  • 产品编号: EC300150
  • 美  元  价: $1369.00
  • 会  员  价: 待定
  • 品       牌: Lucigen
  • 产       地: 美国
  • 公       司: Lucigen
  • 产品分类: ref="." class="index">首页 > Lucigen/TransforMax™ EPI300™ Electrocompetent E. coli/EC300150/50 X 100 µl
  • 公司分类: CRISPR, Large or Difficult Fragment Cloning
:50 X 100 µl
Lucigen/TransforMax™ EPI300™ Electrocompetent E. coli/EC300150/50 X 100 µl
  • Lucigen/TransforMax™ EPI300™ Electrocompetent E. coli/EC300150/50 X 100 µl
商品介绍

Standard cloning for large inserts and Gibson assembly electroporation

  • Formerly from EpicentreClone large inserts and transform large plasmids - up to at least 145 kb plasmid DNA.
  • Generate clones with inducIBLe copy number using CopyControl™ vectors.
  • Achieve high transformation efficiencies: >1 × 1010 cfu/µg pUC19 DNA.
  • Increase Gibson Assembly® colony counts with high-efficiency electroporation
Free Sample Available

Applications

  • Generation of inducIBLe copy-number clones using the CopyControl™ Cloning System.

TransforMax™ EPI300™ E. coli cells lack the tonA gene and are engineered for use with Epicentre's CopyControl™ CDNA, Gene, and PCR Cloning Kit and other CopyControl Cloning Systems* that do not require phage T1-resistant cells. The cells contain an inducIBLe mutant trfA gene whose gene product is required for initiation of replication from the oriV origin of replication, such as that in CopyControl pCC1™ vectors. On LB chloramphenicol plates or in LB or SOC media supplemented with chloramphenicol, CopyControl clones grown in TransforMax EPI300 E. coli replicate at single-copy number from the F-factor replicon because expression of the trfA gene is repressed. Addition of CopyControl Induction Solution induces the cells to express the trfA gene product and induces their replication at high-copy number from oriV (Fig. 1). Replication from oriV results in higher yields and higher purity of cloned DNA.

Benefits

  • Copy-number of clones is under tight control of an inducIBLe promoter linked to the trfA gene.
  • High transformation efficiency with clones of all sizes.
  • lacZΔM15 for blue/white screening of recombinants.
  • Restriction-minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] phenotype enables efficient cloning of methylated DNA.
  • Endonuclease-minus (endA1), to ensure high yields of DNA.
  • Recombination-minus (recA1), for greater stABIlity of large insert clones.

Figure 1. Copy number of CopyControl™ BAC clones can be induced 10- to 20-fold in TransforMax™ EPI300™ E. coli. The yield of BAC DNA from CopyControl BAC clones of 110-145 kb increased >14-fold following addition of CopyControl Induction Solution. U=uninduced cells; I=induced cells.

Genotype

F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG trfA dhfr

TransforMax EPI300 Electrocompetent E. coli

  • Transformation efficiency of >1 x 1010 cfu/µg of pUC19.

TransforMax EPI300 Chemically Competent E. coli

  • Transformation efficiency of >5 x 108 cfu/µg of pUC19.

*Covered by issued and/or pending patents.


ORDER INFORMATION

1,000X concentrated solution. Filter sterilized.
品牌介绍

美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。

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