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公司介绍
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等
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Lucigen/Exonuclease I, E. coli/X40520K/20,000 Units

Lucigen/Exonuclease I, E. coli/X40520K/20,000 Units
  • Lucigen/Exonuclease I, E. coli/X40520K/20,000 Units
商品介绍

DigestssDNA,butnotdsDNA,ina3´to5´direction

  • Epicentre ProductSpecific:ProcessivelydigestssDNAina3´to5´directionwithoutharmingdsDNA-short3´overhangsinDNAarenotdigested
  • FlexIBLe:Activeunderawidevarietyofbufferconditionsandcanbeaddeddirectlyintomostreactionmixes
  • HeatInactivated:Destroytheenzymeactivitybyincubatingat80°Cfor15minutes

ExonucleaseIdigestsssDNAina3´to5´direction,1-3butdoesnotdigestdsDNA.Althoughitrequiresthepresenceofmagnesiumandafree3´-hydroxylterminusforactivity,itisactiveunderawidevarietyofbufferconditionsandcanbeaddeddirectlyintomostreactionmixes.ExonucleaseIcanbeheat-inactivatedbyincubationat80°Cfor15minutes.

Applications

  • RemovalofresidualssDNA,includingoligos,fromreactionmixes.
  • RemovalofssDNAfromnucleicacidmixtures.

UnitDefinition:OneunitofExonucleaseIcatalyzesthereleaseof10nmolofacid-solublenucleotidesfromheat-denaturedcalfthymusDNAin30minutesat37°Cunderstandardassayconditions.

StorageBuffer:50%glycerolcontaining50mMTris-HCl(pH7.5),0.1MNaCl,0.1mMEDTA,1mMDTT,and0.1%Triton®X-100.

QualityControl:ExonucleaseIistestedindegradationofssDNAandisfreeofdetectableRNase,endonuclease,anddouble-strandedexonucleaseactivities.

References

  1. Lehman,I.R.andNussbaum,A.L.(1964)J.Biol.Chem.239,2628.
  2. Kushner,S.R.etal.(1971)Proc.Natl.Acad.Sci.USA68,824.
  3. Kushner,S.R.etal.(1972)Proc.Natl.Acad.Sci.USA69,1366.

Figure1.SpecificityofExonucleaseIforssDNA.200ngofEcoRI-linearizedpUC19dsDNAand1µgofa100-merssDNAoligoweremixedin1XTABufferandincubatedat37°Cfor20minintheabsenceorpresenceof10unitsofExonuclease I.Asseenonthis1%agarosegel,ExonucleaseIcompletelydigestedthelinearssDNAoligo,butleftthelinearizedplasmiddsDNAintact.Lane1,sizeMarkers;Lane2,minusExoI;Lane3,plusExoI.

品牌介绍

美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。


蚂蚁淘(ebiomall)作为一家生命科学领域的垂直电商平台,公司专注生物医学科研用品的全球导购和品牌推广,为海内外厂方与代理经销商搭建跨境贸易系统和交流平台。产品齐全,200+分类,1000+品牌,500万+产品信息。100%正品,专业售后,到货快,在线下单, 简单轻松,节省科学家宝贵的科研时间。为您竭诚服务!

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Lucigen


美国Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。


Lucigen Corporation is working to improve people’s lives by providing quality products and services to life science and healthcare professionals. Founded in Wisconsin in 1998, Lucigen offers products and services that enable life-science professionals to perform their research and testing more efficiently and effectively. The company has grown to manufacture and sell over 325 biomedical research products and services to customers worldwide, all under an ISO 13485 compliant quality system. Core competencies include enzyme evolution, protein expression, cloning, competent cells, next gen sequencing, and molecular diagnostics.


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