请使用支持JavaScript的浏览器! Lucigen-Modifying Enzymes-Lucigen/CircLigase™ ssDNA Ligase/CL4115K/5,000 Units
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公司介绍
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,...
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Lucigen/CircLigase™ ssDNA Ligase/CL4115K/5,000 Units

  • 产品编号: CL4115K
  • 美  元  价: $632.00
  • 会  员  价: 待定
  • 品       牌: Lucigen
  • 产       地: 美国
  • 公       司: Lucigen
  • 产品分类: ref="." class="index">首页 > Lucigen/CircLigase™ ssDNA Ligase/CL4115K/5,000 Units
  • 公司分类: Modifying Enzymes
Size:5,000 Units
Lucigen/CircLigase™ ssDNA Ligase/CL4115K/5,000 Units
  • Lucigen/CircLigase™ ssDNA Ligase/CL4115K/5,000 Units
商品介绍

Thermostable ligase that catalyzes the intramolecular ligation (i.e. circularization) of ssDNA templates

  • Epicentre ProductIntramolecular ligation of single-stranded DNA ends containing 5'-phosphates and 3'-hydroxyl groups
  • ATP-dependent, catalytic enzyme
  • Circularizes ssDNA >30 bases long
  • CompatIBLe with high temperature ligation reactions due to ThermostABIlity of CircLigase™ enzyme

Applications

  • Production of ssDNA templates for rolling-circle replication or rolling-circle transcription experiments.
  • Production of ssDNA templates for RNA polymerase and RNA polymerase inhibitor assays.

CircLigase™ ssDNA Ligase* is a Thermostable ATP-dependent ligase that catalyzes intramolecular ligation (i.e. circularization) of ssDNA templates having a 5´-phosphate and a 3´-hydroxyl group. In contrast to T4 DNA Ligase and Ampligase® DNA Ligase, which ligate DNA ends that are annealed adjacent to each other on a complementary DNA sequence, CircLigase ssDNA Ligase ligates ends of ssDNA in the absence of a complementary sequence. The enzyme is therefore useful for making circular ssDNA molecules from linear ssDNA. Circular ssDNA molecules can be used as substrates for rolling-circle replication or rolling-circle transcription.

Linear ssDNA of >30 bases is circularized by CircLigase enzyme. Under standard reaction conditions, virtually no linear concatamers or circular concatamers are produced. In addition to its activity on ssDNA, CircLigase enzyme also has activity in ligating a single-stranded nucleic acid having a 3´-hydroxyl ribonucleotide and a 5´-phosphorylated ribonucleotide or deoxyribonucleotide.

Unit Definition: One unit of CircLigase enzyme converts 1 pmol of a linear 5´-phosphorylated CircLigase Standard 55-mer Oligo into an exonuclease I-resistant circular form in 1 hour at 60°C under standard assay conditions.

Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.

10X Reaction Buffer: 0.5 M MOPS (pH 7.5), 0.1 M KCl, 50 mM MgCl2, and 10 mM DTT. The Reaction Buffer does not contain
ATP or MnCl2 which must be added to the reaction at final concentrations of 0.05 mM ATP and 2.5 mM MnCl2. A 2.5-mM solution of ATP and a 50-mM solution of MnCl2 are included.

Quality Control: CircLigase ssDNA Ligase is free of detectable phosphatase, DNA exo- and endonuclease, and RNase activities.

Product Citations

  1. Polidoros, A.N. et al. (2006) BioTechniques 41, 35.
  2. Shroff , H. et al. (2005) Nano Letters 5(7), 1509.
  3. Lin, C. et al. (2006) Angewandte Chemie 118, 7699.
  4. Korlach, J. et al. (2008) Proc. Natl. Acad. Sci. USA 105, 1176.
  5. McArthur, M. and Bibb, M.J. (2008) Proc. Natl. Acad. Sci. USA 105, 1020.
  6. Shroff , H. et al. (2008) Biophysical Journal 94, 2179.
  7. Kuhn, H. and Frank-Kamenetskii, M.D. (2008) Nucleic Acids Res. 36, e40.
  8. Murakami, T. et. al. (2008) Nucleic Acids Res. Doi:10.1093/nar/gkn1014

ORDER INFORMATION

CircLigase™ ssDNA Ligase is provided at 100 U/ μl.

Contents: CircLigase™ ssDNA Ligase, CircLigase™ 10X Reaction Buffer, 1 mM ATP, 50 mM MnCl2, CircLigase™ ssDNA Control Oligo, Sterile Water.

品牌介绍

美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。

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