Lucigen/CircLigase™ ssDNA Ligase/CL4115K/5,000 Units

Unit Definition: One unit of CircLigase enzyme converts 1 pmol of a linear 5´-phosphorylated CircLigase Standard 55-mer Oligo into an exonuclease I-resistant circular form in 1 hour at 60°C under standard assay conditions.

Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton^® X-100.

10X Reaction Buffer: 0.5 M MOPS (pH 7.5), 0.1 M KCl, 50 mM MgCl₂, and 10 mM DTT. The Reaction Buffer does not contain
ATP or MnCl₂ which must be added to the reaction at final concentrations of 0.05 mM ATP and 2.5 mM MnCl₂. A 2.5-mM solution of ATP and a 50-mM solution of MnCl₂ are included.

Quality Control: CircLigase ssDNA Ligase is free of detectable phosphatase, DNA exo- and endonuclease, and RNase activities.

Product Citations

1. Polidoros, A.N. et al. (2006) BioTechniques 41, 35.
2. Shroff , H. et al. (2005) Nano Letters 5(7), 1509.
3. Lin, C. et al. (2006) Angewandte Chemie 118, 7699.
4. Korlach, J. et al. (2008) Proc. Natl. Acad. Sci. USA 105, 1176.
5. McArthur, M. and Bibb, M.J. (2008) Proc. Natl. Acad. Sci. USA 105, 1020.
6. Shroff , H. et al. (2008) Biophysical Journal 94, 2179.
7. Kuhn, H. and Frank-Kamenetskii, M.D. (2008) Nucleic Acids Res. 36, e40.
8. Murakami, T. et. al. (2008) Nucleic Acids Res. Doi:10.1093/nar/gkn1014