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Bacterial Expression
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RNA Amplification and Labeling
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Difficult Cloning or BAC/Fosmid Cloning
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General Cloning
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Accessory Kits for Cloning
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General Cloning & Library Construction
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Phage Display Library Applications
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CRISPR, Large or Difficult Fragment Cloning
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Protein Expression
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Low Endotoxin Cells
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DNA Nucleases
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Modifying Enzymes
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RNA Nucleases
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Polymerases
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Reverse Transcriptase Enzymes
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High Yield In Vitro Transcription Kits
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NTP Solutions
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Nuclease Resistant In Vitro Transcription Kits
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NGS Products
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Extraction
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Purification
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Buffers and Reagents
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PCR
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LAMP & Isothermal Amplification
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cDNA Synthesis & RT-PCR
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PCR Accessory Reagents
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Mammalian Expression
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Other
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In Vitro Tn5 Transposon Mutagenesis
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In Vivo Tn5 Transposon Mutagenesis
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TransforMax™ EC100D™pir+andpir-116 ElectrocompetentE. coli
Lucigen/RNase I, E. coli/N6901K/1,000 Units
- 产品编号： N6901K
- 美 元 价： $103.00
- 市 场 价： ¥
- 品 牌： Lucigen
- 产 地： 美国(厂家直采)
- 公 司： Lucigen
- 产品分类： 其他试剂>其他>生化试剂>
- 公司分类： RNA Nucleases
- 联系 Q Q: 3392242852
- 电话号码: 4000-520-616
- 邮 箱: info@ebiomall.com
RemoveunwantedRNAbydigestingwiththisheatlABIle,non-sequencespecificribonuclease
Complete:UnlikeRNaseA,RNaseIdoesnothaveabasepreferenceanddigestsbetweenalldinucleotidepairsinsingle-strandedRNA- HeatLabile:RNaseIiseasilyinactivatedbyheating70°Cfor15minutes
RNaseIdegradessingle-strandedRNAtonucleoside3´monophosphatesvia2´,3´cyclicmonophosphateintermediatesbycleavingbetweenalldinucleotidepairs,2,3unlikeRNaseA,whichcleavesonlyaftercytosineanduridine.Inaddition,theenzymeiscompletelyinactivatedbyheatingat70°Cfor15minutes,eliminatingtherequirementtoremovetheenzymepriortomanysubsequentprocedures.
Applications
- RemovalofRNAfromDNApreparations.1
- RNaseprotectionassaystodetectsingle-basepairmismatchesinRNA:RNAandRNA:DNAhybrids.1
UnitDefinition:OneunitofRNaseIdegrades100ngofE.coliribosomalRNApersecondintoacid-solublenucleotidesat37°Cunderstandardassayconditions.
DilutionandStorageBuffer:50%glycerolcontaining50mMTris-HCl(pH7.5),100mMNaCl,and0.1mMEDTA.
QualityControl:RNaseIisfreeofdetectableexo-andendodeoxyribonucleaseactivities.
References
- Johnson,M.(1996)EpicentreForum3(4),7.
- Shen,V.andSchlessinger,D.(1982)TheEnzymesXV(PartB),501.
- delCardayré,S.B.andRaines,R.T.(1995)Anal.Biochem.225,176.
 |
| Figure1.RemovalofRNAfromplasmidDNApreparations.PlasmidDNAwaspreparedfrom1.5mLofanovernightculture,sUSPendedin50µLTEbuffer(10mMTris-HCl(pH8),1mMEDTA),andtreatedwithRNaseA,RNaseI,ornoenzyme.FiveµLofeachreactionwereresolvedbyagarosegelelectrophoresisandvisualizedbystainingwithethidiumbromide.Lane1,noRNase;Lane2,1.5UofRNaseI;Lane3,20µg/mLRNaseA;MW,1-kbladder.ArrowdenotessmallundigestedRNA. |
ORDERINFORMATION
Providedwithdilutionbuffer,10XTNEbuffer,and0.1MDTT.美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。
厂家直采
正品保障
正规清关
及时交付
总部地址： 江苏省苏州市工业园区星湖街218号 苏州生物医药产业园一期（生物纳米园）A4-216 邮编：215125 工作时间：周一至周五9:00~18:00
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