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公司介绍
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,...
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Lucigen/RNase I, E. coli/N6901K/1,000 Units

  • 产品编号: N6901K
  • 美  元  价: $100.00
  • 会  员  价: 待定
  • 品       牌: Lucigen
  • 产       地: 美国
  • 公       司: Lucigen
  • 产品分类: ref="." class="index">首页 > Lucigen/RNase I, E. coli/N6901K/1,000 Units
  • 公司分类: RNA Nucleases
:1,000 Units
Lucigen/RNase I, E. coli/N6901K/1,000 Units
  • Lucigen/RNase I, E. coli/N6901K/1,000 Units
商品介绍

Remove unwanted RNA by digesting with this heat lABIle, non-sequence specific ribonuclease

  • Epicentre ProductComplete: Unlike RNase A, RNase I does not have a base preference and digests between all dinucleotide pairs in single-stranded RNA
  • Heat LABIle: RNase I is easily inactivated by heating 70°C for 15 minutes

RNase I degrades single-stranded RNA to nucleoside 3´ monophosphates via 2´,3´ cyclic monophosphate intermediates by cleaving between all dinucleotide pairs,2,3 unlike RNase A, which cleaves only after cytosine and uridine. In addition, the enzyme is completely inactivated by heating at 70°C for 15 minutes, eliminating the requirement to remove the enzyme prior to many subsequent procedures.

Applications

  • Removal of RNA from DNA preparations.1
  • RNase protection assays to detect single-basepair mismatches in RNA:RNA and RNA:DNA hybrids.1

Unit Definition: One unit of RNase I degrades 100 ng of E. coli ribosomal RNA per second into acid-soluble nucleotides at 37°C under standard assay conditions.

Dilution and Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, and 0.1 mM EDTA.

Quality Control: RNase I is free of detectable exo- and endodeoxyribonuclease activities.

References

  1. Johnson, M. (1996) Epicentre Forum 3(4), 7.
  2. Shen, V. and Schlessinger, D. (1982) The Enzymes XV (Part B), 501.
  3. delCardayré, S.B. and Raines, R.T. (1995) Anal. Biochem. 225, 176.

 

Figure 1. Removal of RNA from plasmid DNA preparations. Plasmid DNA was prepared from 1.5 mL of an overnight culture, sUSPended in 50 µL TE buffer (10 mM Tris-HCl (pH 8), 1 mM EDTA), and treated with RNase A, RNase I, or no enzyme. Five µL of each reaction were resolved by agarose gel electrophoresis and visualized by staining with ethidium bromide. Lane 1, no RNase; Lane 2, 1.5 U of RNase I; Lane 3, 20 µg/mL RNase A; MW, 1-kb ladder. Arrow denotes small undigested RNA.

ORDER INFORMATION

Provided with dilution buffer, 10X TNE buffer, and 0.1 M DTT.
品牌介绍

美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。

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