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公司介绍
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等
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Lucigen/EconoTaq DNA Polymerase (with Mg++)/30031-3/10,000 U (10 X 1,000 U)

Lucigen/EconoTaq DNA Polymerase (with Mg++)/30031-3/10,000 U (10 X 1,000 U)
  • Lucigen/EconoTaq DNA Polymerase (with Mg++)/30031-3/10,000 U (10 X 1,000 U)
商品介绍
  • GreatTaqPolymeraseperformanceatalowprice.
  • Choiceofreactionbuffers,withorwithoutMgCl2.
  • Non-proofreADIngPolymerase

Free Sample AvailableFreeSampleAvailable

 

 

At$96for1000unitslistprice(quantitydiscountsavailable),EconoTaq’slowpriceiscoupledwithhighqualityandperformance.

QCspecificationsforEconoTaqarerigorous:

  • Greaterthan99%purebySDSgelelectrophoresis(seeFigure1).
  • NodetectableDNAcontaminationasdeterminedbyPCRusinggenericprimers.
  • Nodetectableendonuclease(nicking)activity.Incubationof10UofEconoTaqDNAPolymerasewith1µgofsupercoiledpBR322DNAfor16hoursat70°Cresultsinnodetectableconversiontorelaxedorlinearformsbyagarosegelelectrophoresis.
  • Nodetectableexonucleaseactivity.Incubationof10UofEconoTaqDNAPolymerasewith1µgofHindIII-cutlamBDaDNAfor16hoursat70°Cresultsinnosmearingofbandsonagarosegels.

EconoTaqPerformance
AsshowninFigures2and3Lucigen’sEconoTaqDNAPolymeraseperformsaswellas,orbetterthan,moreexpensiveTaqpreparationsfromseveralothersuppliersinroutinePCR.EconoTaqisaseffectiveinDNAamplificationasanotherstandardPCRenzyme,TflDNApolymerase(Figure4).Inthiscase,thebackgroundofnon-specificamplificationwasmuchlowerwithEconoTaq(compare“+”and“–“lanesforEconoTaqandTflinFigure4).EconoTaqDNAPolymerasealsooffershighlot-to-lotreproducibilityandreliABIlity(Figures2and4)

EconoTaq High Purity

EconoTaq Comparison Promega and NEB

Figure1.HighpurityofEconoTaqDNAPolymerase(SDSPAGE).Lane1,broadrangemolecularweightMarkers;Lane2,LucigenEconoTaqDNAPolymerase

Figure2.TaqDNApolymerasefromPromegaandNewEnglandBiolabswerecomparedtoLucigen’sEconoTaqDNAPolymerase(2differentlots)inamplifyingtheampicillingene(0.8kb)inapUC19vector.(–),noDNA.(+),DNAadded(40ng).MW,1kbladder.

EconoTaq Comparison AmpliTaq

Figure3.EconoTaqvs.AmpliTaq®(AppliedBiosystems)DNApolymeraseingenotyping.AllPCRreactionswereperformedinaRoboCycler96(Stratagene).Hip1genotypingwasperformedusingthefollowingPCRconditions:94°Cfor1min,35cyclesof94°Cfor30sec,62°Cfor60sec,72°Cfor90sec,and72°Cfor7min.Shh,CdoandGas1genotypingwereperformedusingthefollowingPCRconditions:94°Cfor2min,35cyclesof94°Cfor60sec,65°Cfor60sec,72°Cfor90sec,and72°Cfor7min.AllPCRreactionscontainedfinalconcentrationsof1µMofeachprimer,200µMdNTPs,1Xcresolredloadingdye,and1UoftheindicatedTaqpolymerase.SequencesforallPCRprimershavebeenpreviouslypublished(referencesavailable).
DatacourtesyofDr.BenjaminAllen,Dept.Molecular&CellularBIOLOGy,HarvardUniversity.

EconoTaq Tfl Comparison

Figure4.PCRamplificationwasperformedunderstandardconditionsusingthreedifferentlotsofEconoTaqDNAPolymeraseandbuffer,orduplicatereactionswithTflDNApolymeraseandbuffer(Promega).Reactionscontainedprimersspecificforthe16SribosomalRNAgene,withBacillusgenomicDNA(+)ornoDNA(-)asatemplate(1450bpproductexpected).

PleaseNote:
SomeapplicationsinwhichLucigen'sEconoTaqDNAPolymerasecanbeusedmaybecoveredbypatentsissuedandapplicableintheUnitedStatesandcertainothercountries.Becausepurchaseofthisproductdoesnotincludealicensetoperformanypatentedapplication,usersofthisproductmayberequiredtoobtainapatentlicensedependingupontheparticularapplicationinwhichtheproductisused.ThePCRprocessisthesubjectofEuropeanPatentNos.201,184and200,262ownedbyHoffman-LaRoche.ThosepatentsexpiredonMarch28,2006.ThecorrespondingPCRprocesspatentsintheUnitedStatesexpiredonMarch29,2005.Itisthesoleresponsibilityofthebuyertoensurethatuseoftheproductdoesnotinfringethepatentrightsofthirdparties.

ORDERINFORMATION

EconoTaqDNAPolymeraseisprovidedwithachoiceof10XReactionBufferwithMg++(“withMg++”);or10XReactionBufferwithoutMg++andaseparatetubeof25mMMgCl2(“separateMg++”).PleaseinquireforBulkpricing!
品牌介绍

美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。


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