请使用支持JavaScript的浏览器! Lucigen-LAMP & Isothermal Amplification-Lucigen/Sygnis TruePrime™ Single Cell WGA Kit version 2.0/SYG351100/100 rxn
主营:体外转录、基因克隆(BAC克隆试剂盒)、感受态细胞(TG1噬菌体文库构建感受态)、体外转录、转座子突变、蛋白表达
banner
当前位置: 首页 > 产品中心 > LAMP & Isothermal Amplification > Lucigen/Sygnis TruePrime™ Single Cell WGA Kit version 2.0/SYG351100/100 rxn
公司介绍
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,...
问答列表

    暂无问答

Lucigen/Sygnis TruePrime™ Single Cell WGA Kit version 2.0/SYG351100/100 rxn

  • 产品编号: SYG351100
  • 美  元  价: $1890.00
  • 会  员  价: 待定
  • 品       牌: Lucigen
  • 产       地: 美国
  • 公       司: Lucigen
  • 产品分类: ref="." class="index">首页 > Lucigen/Sygnis TruePrime™ Single Cell WGA Kit version 2.0/SYG351100/100 rxn
  • 公司分类: LAMP & Isothermal Amplification
:100 rxn
Lucigen/Sygnis TruePrime™ Single Cell WGA Kit version 2.0/SYG351100/100 rxn
  • Lucigen/Sygnis TruePrime™ Single Cell WGA Kit version 2.0/SYG351100/100 rxn
商品介绍

Single cell whole genome amplification (WGA) with less bias making this kit ideally suited for next generation sequencing applications.

  • High sensitivity: Typical yield is greater than 5 µg when starting with a single mammalian cell.
  • Reduced amplification bias and no primer artefacts: Primer-free amplification method utilizes a combination of TthPrimPol Primase (to synthesize primers) and Phi29 DNA polymerase.
  • Well suited for next generation sequencing: Tested in Illumina and Ion Torrent NGS workflows.
  • Easy and reliable
  • Insensitive to external DNA contamination

  • How does TruePrime™ Technology work?
  • Whole genome amplification from 5 cells
  • Absence of primer artefacts
  • Insensitivity to external DNA contaminations
  • Excellent genome coverage

How does TruePrime technology work?

Single Cell Whole Genome Amplification How It Works

Back to top


Whole genome amplification from 5 cells

Whole Genome Amplification from 5 cells

Back to top


Absence of primer artefacts

TruePRime Single Cell Whole Genome Amplification has absence of artefacts

1 pg of human genomic DNA (~ 1/6 of the content of one human/mammalian cell) has been amplified using either TruePrime™ (TthPrimPol-based MDA) or random primed MDA reactions. Random primed reactions contain 20% of sequences that cannot be mapped to any organism in sequence databases.

Back to top


Insensitivity to external DNA contaminations

Due to the high sensitivity of single cell whole genome amplification techniques there is a considerable danger of contaminating reactions with non-target derived DNA. We therefore recommend to take extreme care when setting up single cell amplification reactions as laid out in the handbook. However, TruePrime™ users have a unique advantage here: TruePrime™ does not accept non-denatured DNA strands as template as reADIly as random primed MDA reactions. Therefore, contaminations coming in from the environment etc. after the denaturation step will not have a strong influence on the composition of your reaction output. We have shown this behaviour in an experiment where we simulate an external DNA contamination by deliberately adding in yeast DNA to a denatured human DNA template. Yeast DNA is in a thousand-fold excess over the target DNA (1 pg human genomic DNA):

TruePrime Single Cell WGA Insensitive to External DNA Contamination

Whereas with TruePrime™ the vast majority of the sequences obtained are target-derived, random primed MDA shows a majority of contaminant-derived sequences.

Back to top


Excellent genome coverage

TruePrime Coverage Yeast Chromosome 7

We have amplified and sequenced the yeast genome using TruePrime™ and Illumina technology. Using 2.5 million read pairs we cover 99.4% of the total yeast genome. Shown is the coverage with narrow width of the distribution curve.

Genome coverage of Yeast

A coverage graph of yeast chromosome 7 exemplifies the more even coverage obtained by TruePrime™ MDA (middle blue line represents mean coverage).

Back to top


ORDER INFORMATION

Each TruePrime™ Single Cell WGA Kit version 2.0 contains: Buffer L1, DTT, Buffer N, Reaction Buffer, dNTPs, Water, Enzyme 1, Enzyme 2, Control 1, and Control 2. The complete manual is available under the Manual tab. Lucigen is an authorized distributor of Sygnis products in the US.

TruePrime™ Single Cell WGA version 2.0 kit is intended for molecular BIOLOGy use only and in vitro use only. This product is not intended for diagnosis, prevention or treatment of a disease in human beings or animals.
品牌介绍

美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。

自营商城图标
厂家直采
全球直采模式 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔
在线客服
客服电话

4000-520-616

微信交流