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主营:体外转录、基因克隆(BAC克隆试剂盒)、感受态细胞(TG1噬菌体文库构建感受态)、体外转录、转座子突变、蛋白表达
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当前位置: 首页 > 产品中心 > cDNA Synthesis & RT-PCR > Lucigen/MessageBOOSTER™ cDNA Synthesis Kit for qPCR/MB060124/24 Reactions
公司介绍
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等
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Lucigen/MessageBOOSTER™ cDNA Synthesis Kit for qPCR/MB060124/24 Reactions

Lucigen/MessageBOOSTER™ cDNA Synthesis Kit for qPCR/MB060124/24 Reactions
  • Lucigen/MessageBOOSTER™ cDNA Synthesis Kit for qPCR/MB060124/24 Reactions
商品介绍

ProduceamplifiedamountsofCDNAfromprecious,limitingtotalRNAsampleswithoutintroducingbias.

  • PerformRNAamplificationonpurifiedtotalRNAfollowedbycDNAsynthesisanddetectionFormerly from Epicentre
  • Amplifywiththishigh-fidelity,linearRNAamplificationprocessthatpreservestherelativetranscriptabundanceofthesample.
  • Getmoredataoutofprecioussamples–uselessRNAformoreRT-qPCRreactions
  • ReADIlyandreproducIBLydetectevenlow-abundancetranscriptsinRNAfromasinglecell(CTvalues<35cycles)
  • CollectsmallRNAsampleslessoften.
  • FastprotocolamplifiesandsynthesizescDNAinonly1day.
Applications
  • SignificantlyincreasingthenumberofqRT-PCRsthatcanbeobtainedfromverysmallamountsoftotalRNA,aslittleasonecell(10pg).
  • GenerationoflargeamountsofcDNAfromverysmallsamplesofintacttotalRNAforarchivalpurposes.

TheMessageBOOSTER™cDNASynthesisKitforqPCRenablestheusertoperformsensitiveqPCRamplificationsusingthetotalRNAfromverysmallpopulationsofcells,evenfromaslittleasonecell(Table1).

ThekitamplifiesthemRNAcontainedinasmalltotalRNAsampleandthenconvertstheamplifiedRNAtocDNAthatisreadyforPCRorqPCR(Fig.1).TheMessageBOOSTERcDNASynthesisKitforqPCRusesoligo(dT)toprimecDNAsynthesis.Therefore,thiskitisbestusedwithintacttotalRNApreparations.

Figure1(clicktoenlarge).OverviewoftheMessageBOOSTER™cDNASynthesisKitprocedure.ThekitusesalinearRNAamplificationprocesstoamplifytheRNAfromasmallpopulationofcells,thenconvertstheamplifiedRNAtocDNAthatisreadyforPCR.Theprocedurecanbecompletedin1day.

Figure2.SensitivityoftheMessageBOOSTER™cDNASynthesisKit.cDNAproducedfrom10pgoftotalNRKRNAsignificantlyimprovedthesensitivity(loweredtheCT)ofdetectingthelow-abundancePBGDtranscriptcomparedtocDNAproducedfrom10pgofunamplifiedRNA.

AmountofTotalRNAinaMessageBOOSTER™ReactionNumberofqPCRsThatCanBePerformed
Low-toMedium-AbundanceTranscriptsaMedium-toHigh-AbundanceTranscriptsa
10pg(~1cell)≥10≥100
100pg(~10cells)≥100≥1,000
500pg(~50cells)≥500-1,000≥5,000-10,000
Table1.ThenumberofqPCRamplificationsthatcanbeperformedusingcDNAproducedbyaMessageBOOSTER™cDNASynthesisKit.ThenumberofqPCRsisdependentontheamountofinputtotalRNAandtheabundanceofthetranscript(s)ofinterest.

a.Low-AbundanceTranscripts=1-1,000copiespercell
Medium-AbundanceTranscripts=1,000-10,000copiespercell
High-AbundanceTranscripts=10,000-100,000copiespercell

ProductCitations

  1. Grunenwald,H.etal.(2006)EpicentreForum13(3),22.
  2. DeFazio,R.A.etal.(2006)J.Neuroscience26,3971.
  3. Kaeffer,B.etal.(2007)PediatricResearch62,564.
  4. Lochner,M.,etal.(2008)J.Exp.Med.205,1381.
  5. Graham,D.M.etal.(2008)J.Neurophysiology,99,2522.
  6. Liu,X.et.al.(2009)Am.J.Physiol.LungCellMolPhysiol.296,L158
  7. Yakovlev,I.A.etal.(2008)FungalGeneticandBIOLOGy45,498
  8. Jung,Y.etal.(2008)StemCellsExpress10,1634
  9. Xi,D.etal.(2008)J.NeurosciMethod10,1016
  10. Michel,M-L.etal.(2008)Proc.Nat'lAcadSci-USA105(50),19845
  11. Satoh-Takayama,N.etal.(2008)Immunity29,958
  12. Bouskra,D.etal.(2008)Nature10,1038
  13. Yakovlev,I.A.etal.(2008)FungalGeneticsandBiology45,498
  14. Roberts,C.D.et.al.(2009)J.Physiology587,1657
  15. Peduto,L.et.al.(2009)J.Immunology182,5789
  16. Xi,D.et.al.(2009)Int'lJ.Neuropsychopharmacology,May13:1-14

ORDERINFORMATION

MessageBOOSTER™T7-Oligo(dT)PrimerA,RNaseInhibitor,MessageBOOSTER™ReverseTranscriptionPreMix,MessageBOOSTER™DNAPolymerasePreMix1,MessageBOOSTER™DNAPolymerase1,MessageBOOSTER™cDNAFinishingSolution,MessageBOOSTER™RandomPrimers,MessageBOOSTER™RNaseH,MMLVReverseTranscriptase,MessageBOOSTER™InVitroTranscriptionPreMixA,MessageBOOSTER™T7RNAPolymerase,MessageBOOSTER™T7TranscriptionBuffer,RNase-FreeDNaseI,ForwardandReverseControlPCRPrimers,DTT,RNase-FreeWater,Poly(I),NRKTotalRNAControl.
品牌介绍

美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。


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英国LGC(Laboratory of the Government Chemist政府化学家实验室)有限公司成立于1842年,是集实验室服务、测量标准、标准物质及实验室能力验证于一体的市场领导者。历经170年,LGC有限公司现已发展成集团公司,技术范围涵盖:研发及质量控制;医药及生物技术;刑侦科学;生命科学;食物链及环境监督安全等基础研究领域。基因组学2大品牌:Lucigen 和Epicentre

美国Lucigen公司,自1998年成立至今,Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,Lucigen产品清单:

货号

名称

规格

14000-1

NxSeq® AmpFREE Low DNA Library Kit DNA建库试剂盒

12 Reactions

14000-2

NxSeq® AmpFREE Low DNA Library Kit DNA建库试剂盒

48 Reactions

14300-1

NxSeq® Adaptors, Box 1 建库接头

12*4 rxns

14400-1

NxSeq® Adaptors, Box 2 建库接头

12*4 rxns

15012-1

NxSeq® UltraLow DNA Library Kit DNA文库制备试剂盒

12 Reactions

15096-1

NxSeq® UltraLow DNA Library Kit 二代测序DNA建库试剂盒

96 Reactions

15100-1

NxSeq® Single Indexing Kit, Set A

12*4 rxns

15200-1

NxSeq® Single Indexing Kit, Set B

12*4 rxns

15300-1

NxSeq® HT Dual Indexing Kit

96 rxns


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