请使用支持JavaScript的浏览器! Lucigen-In Vitro Tn5 Transposon Mutagenesis-Lucigen/EZ-Tn5™ <R6Kyori/KAN-2> Insertion Kit/EZI011RK/10 Reactions
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公司介绍
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,...
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Lucigen/EZ-Tn5™ <R6Kyori/KAN-2> Insertion Kit/EZI011RK/10 Reactions

  • 产品编号: EZI011RK
  • 美  元  价: $527.00
  • 会  员  价: 待定
  • 品       牌: Lucigen
  • 产       地: 美国
  • 公       司: Lucigen
  • 产品分类: ref="." class="index">首页 > Lucigen/EZ-Tn5™ <R6Kyori/KAN-2> Insertion Kit/EZI011RK/10 Reactions
  • 公司分类: In Vitro Tn5 Transposon Mutagenesis
Size:10 Reactions
Lucigen/EZ-Tn5™ <R6Kyori/KAN-2> Insertion Kit/EZI011RK/10 Reactions
  • Lucigen/EZ-Tn5™ <R6Kyori/KAN-2> Insertion Kit/EZI011RK/10 Reactions
商品介绍

Random insertion of R6K origin for rescue of flanking DNA or plasmids

  • Insert R6K origin randomly into any DNA sequence in vitro Formerly from Epicentre
  • Skip primer walking - simplify Sanger sequencing of large DNA inserts
  • Rescue clones in E. coli host expressing the pir gene product
  • Minimize insertion bias with the hyperactive Tn5 system, known for highest level of randomness

Applications

  • "Rescue" of plasmids or any other circularized DNA (e.g., mitochondrial DNA) that would not otherwise replicate in E. coli because they lack a recognizable origin of replication and/or a selectable Marker.
  • Preparation of DNA sequencing templates from transposon insertion clones without primer walking or additional subcloning.
  • Creation of a library of random gene knockouts in vitro to facilitate genetic analysis of plasmid-encoded genes.

The EZ-Tn5™ <R6Kγori/KAN-2> Insertion Kit* facilitates the sequencing and genetic analysis of plasmids or any other circularized DNA that would not otherwise replicate in E. coli.1,2 The kit is based upon the EZ-Tn5™ <R6Kγori/KAN-2> Transposon which carries the E. coli R6Kγ conditional origin of replication (R6Kγori) and a kanamycin resistance Marker. A simple, one-step, 2-hour in vitro reaction randomly inserts the transposon into the target DNA. Then an aliquot of the reaction is used to transform an E. coli host expressing the pir gene product, which is required for replication from the R6Kγori. Insertion clones are selected on kanamycin plates and can be sequenced bidirectionally using the provided primers that are homologous to the ends of the transposon. Clones can be maintained in Epicentre's TransforMax™ EC100D™ pir+ or TransforMax™ EC100D™ pir-116 strains.3

g_eztn5_r6kgori_kan2_insertion Figure 1. A plasmid containing the EZ-Tn5™ <R6Kγori/KAN-2> Transposon can be maintained in TransforMax™ EC100D™ pir+ cells at ~15 copies per cell (Lanes 1-4) or TransforMax™ EC100D™ pir-116 cells at ~250 copies per cell (Lanes 5-8). Colonies from randomly chosen clones were processed using the Colony Fast-Screen™ Kit. A 5-microliter aliquot of lysate was loaded per lane. M, supercoiled DNA ladder.

References

  1. Jendrisak, J. et al. (2002) Epicentre Forum 9(1), 14.
  2. Yoon, Y. and Koob, M. (2003) Epicentre Forum 10(2), 10.
  3. Metcalf, W.W. et al. (1994) Gene 138, 1.

*Covered by issued and/or pending patents, exclusively licensed or assigned to Epicentre® (an Illumina® Company).

ORDER INFORMATION

EZ-Tn5™ Transposase, EZ-Tn5™ <R6Kyori/KAN-2> Transposon, EZ-Tn5™ 10X Reaction Buffer, EZ-Tn5™ 10X Stop Solution, Forward and Reverse Primers, Control Target DNA, Sterile Water.
品牌介绍

美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。

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