请使用支持JavaScript的浏览器! Lucigen-In Vitro Tn5 Transposon Mutagenesis-Lucigen/EZ-Tn5™ <T7/KAN-2> Promoter Insertion Kit/EZI03T7/10 Reactions
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公司介绍
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,...
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Lucigen/EZ-Tn5™ <T7/KAN-2> Promoter Insertion Kit/EZI03T7/10 Reactions

  • 产品编号: EZI03T7
  • 美  元  价: $527.00
  • 会  员  价: 待定
  • 品       牌: Lucigen
  • 产       地: 美国
  • 公       司: Lucigen
  • 产品分类: ref="." class="index">首页 > Lucigen/EZ-Tn5™ <T7/KAN-2> Promoter Insertion Kit/EZI03T7/10 Reactions
  • 公司分类: In Vitro Tn5 Transposon Mutagenesis
Size:10 Reactions
Lucigen/EZ-Tn5™ <T7/KAN-2> Promoter Insertion Kit/EZI03T7/10 Reactions
  • Lucigen/EZ-Tn5™ <T7/KAN-2> Promoter Insertion Kit/EZI03T7/10 Reactions
商品介绍

Random insertion of T7 promoter to drive gene expression with T7 RNA polymerase

  • Insert T7 promoter randomly into any DNA sequence in vitro Formerly from Epicentre
  • Isolate positive clones with the selectable kanamycin Marker
  • Express resulting products in vivo or in vitro
  • Minimize insertion bias with the hyperactive Tn5 system, known for highest level of randomness

Applications

  • Random insertion of a single T7 RNA Polymerase promoter to synthesize RNA from any region of cloned DNA.

The EZ-Tn5™ <T7/KAN-2> Promoter Insertion Kit* provides an easy and reliable method to randomly insert a transposon containing a phage T7 RNA polymerase promoter into any DNA molecule in vitro. The transposon end has no termination sequences, so RNA can be produced from chosen insertion clones by in vitro transcription from the T7 RNA polymerase promoter using, for example, any of Epicentre's T7 RNA Polymerase Transcription Kits. RNA can also be generated for in vivo expression studies in cells having an inducIBLe T7 RNA polymerase gene.


Figure 1 (click to enlarge). RNA transcripts can be produced from insertion clones in vitro or in vivo using the EZ-Tn5™ <T7/KAN-2> Promoter Insertion Kit.

*Covered by issued and/or pending patents, exclusively licensed or assigned to Epicentre® (an Illumina® Company).

ORDER INFORMATION

Contents: EZ-Tn5™ Transposase, EZ-Tn5™ Transposon, EZ-Tn5™ 10X Reaction Buffer, EZ-Tn5™ 10X Stop Solution, Forward and Reverse Primers, Control Target DNA, Sterile Water
品牌介绍

美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。

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