请使用支持JavaScript的浏览器! Lucigen-In Vitro Tn5 Transposon Mutagenesis-Lucigen/EZ-Tn5™ Transposase/TNP92110/10 Units
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公司介绍
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,...
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Lucigen/EZ-Tn5™ Transposase/TNP92110/10 Units

  • 产品编号: TNP92110
  • 美  元  价: $419.00
  • 会  员  价: 待定
  • 品       牌: Lucigen
  • 产       地: 美国
  • 公       司: Lucigen
  • 产品分类: ref="." class="index">首页 > Lucigen/EZ-Tn5™ Transposase/TNP92110/10 Units
  • 公司分类: In Vitro Tn5 Transposon Mutagenesis
Size:10 Units
Lucigen/EZ-Tn5™ Transposase/TNP92110/10 Units
  • Lucigen/EZ-Tn5™ Transposase/TNP92110/10 Units
商品介绍
  • Create custom transposomes for in vitro transposomics Formerly from Epicentre
  • Integrate selectable Markers, promoters or replication origins into DNA in vitro with EZ-Tn5 insertion kits

Applications

  • In vitro insertion of an EZ-Tn5 Transposon into DNA cloned in vectors, such as plasmids, fosmids, cosmids, or BACs as well as in vitro insertion into linear DNA.*
  • Preparation of EZ-Tn5 Transposomes for in vivo transposition following electroporation into living cells.**

EZ-Tn5™ Transposase is a hyperactive form of Tn5 transposase.1 The highly purified, single-subunit enzyme can be used to randomly insert (transpose or "hop") any EZ-Tn5 Transposon into any target DNA in vitro with an efficiency up to >106 insertion clones per standard reaction. When incubated with an EZ-Tn5 Transposon in the absence of Mg2+, a stable EZ-Tn5 Transposome™ complex is formed. The Transposome is so stable that it can be electroporated into living cells. Once in the cell, the Transposome is activated by intracellular Mg2+ and the EZ-Tn5 Transposon component is randomly inserted into the host's genomic DNA. The three-dimensional structure of a Transposome complex has been elucidated.2

A typical EZ-Tn5 transposition reaction requires four components: (1) the EZ-Tn5 Transposase; (2) an EZ-Tn5 Transposon; (3) a target DNA; and (4) the presence of Mg2+. The highly random insertion of an EZ-Tn5 Transposon into the target DNA3 proceeds by a cut-and-paste mechanism,1 catalyzed by the EZ-Tn5 Transposase, and results in a 9-bp duplication of target DNA sequence immediately adjacent to both ends of the Transposon.

Benefits

  • Specifically and uniquely recognizes the Outer End sequences of naturally occurring Tn5 and mini-Tn5 transposons and the hyperactive Mosaic Ends of EZ-Tn5 Transposons.

References

  1. Goryshin, I.Y. and Reznikoff , W.S. (1998) J. Biol. Chem. 273, 7367.
  2. Davies, D.R. et al. (2000) Science 289, 77.
  3. Shevchenko, Y. et al. (2002) Nucleic Acids Res. 30, 2469.

*Covered by issued and/or pending patents, exclusively licensed or assigned to Epicentre® (an Illumina® Company).
**The use of Transposome™ complexes for
in vivo insertion of a transposon, including, but not limited, to HyperMu™ and EZ-Tn5™ Transposome™ complexes, is covered by U.S. Patent No. 6,159,736 and related patents and patent applications, exclusively licensed to Epicentre® (an Illumina® Company).

ORDER INFORMATION

10 units of transposase is enough enzyme for 10 standard reactions.

Please note: Illumina requires that a Transposome™ License Acknowledgement is signed prior to processing orders for EZ-Tn5™ Transposase. Please complete this form before submitting your online order for this item.

品牌介绍

美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。

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