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当前位置: 首页 > 产品中心 > In Vitro Tn5 Transposon Mutagenesis > Lucigen/EZ-Tn5™ Transposase/TNP92110/10 Units
公司介绍
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等
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Lucigen/EZ-Tn5™ Transposase/TNP92110/10 Units

Lucigen/EZ-Tn5™ Transposase/TNP92110/10 Units
  • Lucigen/EZ-Tn5™ Transposase/TNP92110/10 Units
商品介绍
  • CreatecustomtransposomesforinvitrotransposomicsFormerly from Epicentre
  • IntegrateselectableMarkers,promotersorreplicationoriginsintoDNAinvitrowithEZ-Tn5insertionkits

Applications

  • InvitroinsertionofanEZ-Tn5TransposonintoDNAclonedinvectors,suchasplasmids,fosmids,cosmids,orBACsaswellasinvitroinsertionintolinearDNA.*
  • PreparationofEZ-Tn5Transposomesforinvivotranspositionfollowingelectroporationintolivingcells.**

EZ-Tn5™TransposaseisahyperactiveformofTn5transposase.1Thehighlypurified,single-subunitenzymecanbeusedtorandomlyinsert(transposeor"hop")anyEZ-Tn5TransposonintoanytargetDNAinvitrowithanefficiencyupto>106insertionclonesperstandardreaction.WhenincubatedwithanEZ-Tn5TransposonintheabsenceofMg2+,astableEZ-Tn5Transposome™complexisformed.TheTransposomeissostablethatitcanbeelectroporatedintolivingcells.Onceinthecell,theTransposomeisactivatedbyintracellularMg2+andtheEZ-Tn5Transposoncomponentisrandomlyinsertedintothehost'sgenomicDNA.Thethree-dimensionalstructureofaTransposomecomplexhasbeenelucidated.2

AtypicalEZ-Tn5transpositionreactionrequiresfourcomponents:(1)theEZ-Tn5Transposase;(2)anEZ-Tn5Transposon;(3)atargetDNA;and(4)thepresenceofMg2+.ThehighlyrandominsertionofanEZ-Tn5TransposonintothetargetDNA3proceedsbyacut-and-pastemechanism,1catalyzedbytheEZ-Tn5Transposase,andresultsina9-bpduplicationoftargetDNAsequenceimmediatelyadjacenttobothendsoftheTransposon.

Benefits

  • SpecificallyanduniquelyrecognizestheOuterEndsequencesofnaturallyoccurringTn5andmini-Tn5transposonsandthehyperactiveMosaicEndsofEZ-Tn5Transposons.

References

  1. Goryshin,I.Y.andReznikoff,W.S.(1998)J.Biol.Chem.273,7367.
  2. Davies,D.R.etal.(2000)Science289,77.
  3. Shevchenko,Y.etal.(2002)NucleicAcidsRes.30,2469.

*Coveredbyissuedand/orpendingpatents,exclusivelylicensedorassignedtoEpicentre®(anIllumina®Company).
**TheuseofTransposome™complexesfor
invivoinsertionofatransposon,including,butnotlimited,toHyperMu™andEZ-Tn5™Transposome™complexes,iscoveredbyU.S.PatentNo.6,159,736andrelatedpatentsandpatentapplications,exclusivelylicensedtoEpicentre®(anIllumina®Company).

ORDERINFORMATION

10unitsoftransposaseisenoughenzymefor10standardreactions.

Pleasenote:IlluminarequiresthataTransposome™LicenseAcknowledgementissignedpriortoprocessingordersforEZ-Tn5™Transposase.Pleasecompletethisformbeforesubmittingyouronlineorderforthisitem.

品牌介绍

美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。

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