请使用支持JavaScript的浏览器!
主营:体外转录、基因克隆(BAC克隆试剂盒)、感受态细胞(TG1噬菌体文库构建感受态)、体外转录、转座子突变、蛋白表达
banner
当前位置: 首页 > 产品中心 > In Vivo Tn5 Transposon Mutagenesis > Lucigen/EZ-Tn5™ <R6Kyori/KAN-2>Tnp Transposome™ Kit/TSM08KR/10 Reactions
公司介绍
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等
问答列表

    暂无问答

Lucigen/EZ-Tn5™ <R6Kyori/KAN-2>Tnp Transposome™ Kit/TSM08KR/10 Reactions

Lucigen/EZ-Tn5™ <R6Kyori/KAN-2>Tnp Transposome™ Kit/TSM08KR/10 Reactions
  • Lucigen/EZ-Tn5™ <R6Kyori/KAN-2>Tnp Transposome™ Kit/TSM08KR/10 Reactions
商品介绍

Generaterandomgeneknockoutsinnon-E.colibacteria

  • GeneratemutantswithimprovedgeneticsorfunctionFormerly from Epicentre
  • Identifygenesinvolvedinpathogenesis,toxicity,biofilmdevelopment
  • Unravelmetabolicpathways
  • Identifyessentialgenesandregulatoryelements
  • Characterizenovelgenesandgenefunctions
  • InsertKanselectableMarkerintogDNAandrescueclonesinE.colihostexpressingthepirgeneproduct
  • 100’sofcitationsformanydifferentapplications

Applications

  • Rescuecloningoftransposon-mutagenizedmicrobialgenes(Fig.1).
  • Rescueofplasmidsfromnon-E.colibacteria.

AmongtheadvantagesoftransposonmutagenesisisthatthetransposonservesasamarkerthatcanbeusedtocloneandsequencetheregionofgenomicDNAthathasbeendisrupted.NothingmakesthiscloningprocesseasierthancreatingmutationsinvivowiththeEZ-Tn5™<R6Kγori/KAN-2>TnpTransposome™Kit.*Inadditiontoencodingabroadhost-rangekanamycinresistancegene,thetransposoncontainsanE.coliconditionaloriginofreplication(R6Kγori).Thepresenceofthisoriginofreplicationenablesthepropagationor"rescue"oftheregionofgenomicDNA,orplasmid,intowhichthetransposonhasbeeninserted.

First,electroporatetheTransposomeintoelectrocompetentcellsofthehighestpossIBLetransformationefficiency.ActivationoftheTransposomebyMg2+inthecellresultsintherandominsertionoftheEZ-Tn5<R6Kγori/KAN-2>Transposonintothehost'sgenomicDNA.Selecttranspositionclonesonkanamycinplatesorbyscreeningforthelossofgenefunction.

Benefits

  • EasilyrecoverandpropagateplasmidsfromdiversebacterialgenerathatwillnotnormallyreplicateinE.coli.
  • Simplerescuecloningprocessofmutagenizedgenesspeedsupstructure/functionstudiesandsequencing.
  • RandominsertionoftransposonDNAassuresexcellentcoverageofentirebacterialchromosome.
  • Rescueclonescanbesequencedbidirectionallyusingtheprimersprovidedthatarehomologoustotheendsofthetransposon.

Figure1(clicktoenlarge).TheprocessforrescuecloningoftransposoninsertionsitesingenomicDNAusingtheEZ-Tn5™<R6Kγori/KAN-2>TnpTransposome™andTransforMax™EC100D™pir+orTransforMax™EC100D™pir-116ElectrocompetentE.coli.

*Coveredbyissuedand/orpendingpatents,exclusivelylicensedorassignedtoEpicentre®(anIllumina®Company).

ORDERINFORMATION

EZ-Tn5™<R6Kyori/KAN-2>TnpTransposome,KAN-2FP-1ForwardPrimer,R6KAN-2RP-1ReversePrimer,SterileWater.
品牌介绍

美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。

自营商城图标
厂家直采
全球直采模式 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔
在线客服
客服电话

4000-520-616

0512-67156496