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公司介绍
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,...
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Lucigen/EZ-Tn5™ <R6Kyori/KAN-2>Tnp Transposome™ Kit/TSM08KR/10 Reactions

  • 产品编号: TSM08KR
  • 美  元  价: $521.00
  • 会  员  价: 待定
  • 品       牌: Lucigen
  • 产       地: 美国
  • 公       司: Lucigen
  • 产品分类: ref="." class="index">首页 > Lucigen/EZ-Tn5™ <R6Kyori/KAN-2>Tnp Transposome™ Kit/TSM08KR/10 Reactions
  • 公司分类: In Vivo Tn5 Transposon Mutagenesis
Size:10 Reactions
Lucigen/EZ-Tn5™ <R6Kyori/KAN-2>Tnp Transposome™ Kit/TSM08KR/10 Reactions
  • Lucigen/EZ-Tn5™ <R6Kyori/KAN-2>Tnp Transposome™ Kit/TSM08KR/10 Reactions
商品介绍

Generate random gene knockouts in non-E. coli bacteria

  • Generate mutants with improved genetics or functionFormerly from Epicentre
  • Identify genes involved in pathogenesis, toxicity, biofilm development
  • Unravel metabolic pathways
  • Identify essential genes and regulatory elements
  • Characterize novel genes and gene functions
  • Insert Kan selectable Marker into gDNA and rescue clones in E. coli host expressing the pir gene product
  • 100’s of citations for many different applications

Applications

  • Rescue cloning of transposon-mutagenized microbial genes (Fig. 1).
  • Rescue of plasmids from non-E. coli bacteria.

Among the advantages of transposon mutagenesis is that the transposon serves as a Marker that can be used to clone and sequence the region of genomic DNA that has been disrupted. Nothing makes this cloning process easier than creating mutations in vivo with the EZ-Tn5™ <R6Kγori/KAN-2>Tnp Transposome™ Kit.* In addition to encoding a broad host-range kanamycin resistance gene, the transposon contains an E. coli conditional origin of replication (R6Kγori). The presence of this origin of replication enables the propagation or "rescue" of the region of genomic DNA, or plasmid, into which the transposon has been inserted.

First, electroporate the Transposome into electrocompetent cells of the highest possIBLe transformation efficiency. Activation of the Transposome by Mg2+ in the cell results in the random insertion of the EZ-Tn5 <R6Kγori/KAN-2> Transposon into the host's genomic DNA. Select transposition clones on kanamycin plates or by screening for the loss of gene function.

Benefits

  • Easily recover and propagate plasmids from diverse bacterial genera that will not normally replicate in E. coli.
  • Simple rescue cloning process of mutagenized genes speeds up structure/function studies and sequencing.
  • Random insertion of transposon DNA assures excellent coverage of entire bacterial chromosome.
  • Rescue clones can be sequenced bidirectionally using the primers provided that are homologous to the ends of the transposon.

Figure 1 (click to enlarge). The process for rescue cloning of transposon insertion sites in genomic DNA using the EZ-Tn5™ <R6Kγori/KAN-2>Tnp Transposome™ and TransforMax™ EC100D™ pir+ or TransforMax™ EC100D™ pir-116 Electrocompetent E. coli.

*Covered by issued and/or pending patents, exclusively licensed or assigned to Epicentre® (an Illumina® Company).

ORDER INFORMATION

EZ-Tn5™ <R6Kyori/KAN-2>Tnp Transposome, KAN-2 FP-1 Forward Primer, R6KAN-2 RP-1 Reverse Primer, Sterile Water.
品牌介绍

美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。

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