请使用支持JavaScript的浏览器! Lucigen-In Vivo Tn5 Transposon Mutagenesis-Lucigen/TypeOne™ Restriction Inhibitor/TY0261H/100 µg
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公司介绍
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,...
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Lucigen/TypeOne™ Restriction Inhibitor/TY0261H/100 µg

  • 产品编号: TY0261H
  • 美  元  价: $178.00
  • 会  员  价: 待定
  • 品       牌: Lucigen
  • 产       地: 美国
  • 公       司: Lucigen
  • 产品分类: ref="." class="index">首页 > Lucigen/TypeOne™ Restriction Inhibitor/TY0261H/100 µg
  • 公司分类: In Vivo Tn5 Transposon Mutagenesis
Size:100 µg
Lucigen/TypeOne™ Restriction Inhibitor/TY0261H/100 µg
  • Lucigen/TypeOne™ Restriction Inhibitor/TY0261H/100 µg
商品介绍

Better transformation efficiencies with restriction system inhibition

  • Formerly from EpicentreIncrease the insertion efficiency of EZ-Tn5™ Transposome™ complexes.
  • Enhance the transformation efficiency of unmodified DNA by blocking type I R-M systems, which are widespread in Eubacteria and Archaebacteria.

Applications

  • Increase the transformation efficiency of unmodified DNA2 by blocking type I R-M systems, which are widespread in Eubacteria and Archaebacteria.3
  • Increase the insertion efficiency of EZ-Tn5™ Transposome™ complexes.2

DNA transformation can be difficult to achieve in many bacterial strains due to the presence of one or more restriction and modification (R-M) systems that cleave unmodified DNA that is recognized as "foreign." TypeOne™ Restriction Inhibitor* provides a powerful method for increasing transformation efficiencies in bacterial strains with type I R-M systems. Developed as a unique preparation of a phage protein called ocr,1 TypeOne Inhibitor is reADIly electroporated into cells along with transforming DNA. In vivo, the protein acts as a molecular decoy that blocks the DNA-binding site of type I R-M enzymes and inhibits DNA cleavage.

Benefits

  • Easy to use: Simply add TypeOne Restriction Inhibitor to unmodified DNA or a Transposome and electroporate using standard methods.
  • Blocks all known families of type I R-M enzymes1 and can therefore be used to increase transformation efficiencies in a variety of bacterial cells.

Table 1. Effect of TypeOne™ Restriction Inhibitor on transformation efficiencies.

Strain (Type I R-M system)

TypeOne™
Inhibitor
Type of DNA or Transposome™ Recombinants
per µg DNA
S. typhimurium LT2 (StyL TIII) pUC19 (100 pg) 3.0 × 106
S. typhimurium LT2 (StyL TIII) X pUC19 (100 pg) 3.0 × 108
E. coli MG1655 (EcoK1) 48 kb fosmid (50 ng) 3.0 × 103
E. coli MG1655 (EcoK1) X 48 kb fosmid (50 ng) 1.4 × 106
S. typhimurium LT2 (StyL TIII) EZ-Tn5™ <R6Kγori/KAN-2>Tnp Transposome™ (1 µl) 1.3 × 104
S. typhimurium LT2 (StyL TIII) X EZ-Tn5™ <R6Kγori/KAN-2>Tnp Transposome™ (1 µl) 1.0 × 106

References

  1. Walkinshaw, M.D. et al. (2002) Molec. Cell 9, 187.
  2. Hoff man, L. et al. (2002) Epicentre Forum 9(2), 8.
  3. Murray, N.E. et al. (2000) Microbial. Molec. Biol. Rev. 64, 412.

*Covered by issued and/or pending patents, exclusively licensed or assigned to Epicentre® (an Illumina® Company).

品牌介绍

美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。

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