Lucigen/TypeOne™ Restriction Inhibitor/TY0261H/100 µg

Applications

• Increase the transformation efficiency of unmodified DNA² by blocking type I R-M systems, which are widespread in Eubacteria and Archaebacteria.³
• Increase the insertion efficiency of EZ-Tn5™ Transposome™ complexes.²

DNA transformation can be difficult to achieve in many bacterial strains due to the presence of one or more restriction and modification (R-M) systems that cleave unmodified DNA that is recognized as "foreign." TypeOne™ Restriction Inhibitor* provides a powerful method for increasing transformation efficiencies in bacterial strains with type I R-M systems. Developed as a unique preparation of a phage protein called ocr,¹ TypeOne Inhibitor is reADIly electroporated into cells along with transforming DNA. In vivo, the protein acts as a molecular decoy that blocks the DNA-binding site of type I R-M enzymes and inhibits DNA cleavage.

Benefits

• Easy to use: Simply add TypeOne Restriction Inhibitor to unmodified DNA or a Transposome and electroporate using standard methods.
• Blocks all known families of type I R-M enzymes¹ and can therefore be used to increase transformation efficiencies in a variety of bacterial cells.

Table 1. Effect of TypeOne™ Restriction Inhibitor on transformation efficiencies.

References

1. Walkinshaw, M.D. et al. (2002) Molec. Cell 9, 187.
2. Hoff man, L. et al. (2002) Epicentre Forum 9(2), 8.
3. Murray, N.E. et al. (2000) Microbial. Molec. Biol. Rev. 64, 412.

*Covered by issued and/or pending patents, exclusively licensed or assigned to Epicentre® (an Illumina® Company).