>
产品中心 >
In Vivo Tn5 Transposon Mutagenesis >
Lucigen/TypeOne™ Restriction Inhibitor/TY0261H/100 µg
-
Bacterial Expression
-
RNA Amplification and Labeling
-
Difficult Cloning or BAC/Fosmid Cloning
-
General Cloning
-
Accessory Kits for Cloning
-
General Cloning & Library Construction
-
Phage Display Library Applications
-
CRISPR, Large or Difficult Fragment Cloning
-
Protein Expression
-
Low Endotoxin Cells
-
DNA Nucleases
-
Modifying Enzymes
-
RNA Nucleases
-
Polymerases
-
Reverse Transcriptase Enzymes
-
High Yield In Vitro Transcription Kits
-
NTP Solutions
-
Nuclease Resistant In Vitro Transcription Kits
-
NGS Products
-
Extraction
-
Purification
-
Buffers and Reagents
-
PCR
-
LAMP & Isothermal Amplification
-
cDNA Synthesis & RT-PCR
-
PCR Accessory Reagents
-
Mammalian Expression
-
Other
-
In Vitro Tn5 Transposon Mutagenesis
-
In Vivo Tn5 Transposon Mutagenesis
-
TransforMax™ EC100D™pir+andpir-116 ElectrocompetentE. coli
暂无问答
Lucigen/TypeOne™ Restriction Inhibitor/TY0261H/100 µg
- 产品编号： TY0261H
- 美 元 价： $182.00
- 市 场 价： ¥3640.00
- 品 牌： Lucigen
- 产 地： 美国(厂家直采)
- 公 司： Lucigen
- 产品分类： 转染试剂>基因编辑>分子类>
- 公司分类： In Vivo Tn5 Transposon Mutagenesis
- 联系 Q Q: 3392242852
- 电话号码: 4000-520-616
- 邮 箱: info@ebiomall.com
Bettertransformationefficiencieswithrestrictionsysteminhibition
IncreasetheinsertionefficiencyofEZ-Tn5™Transposome™complexes.- EnhancethetransformationefficiencyofunmodifiedDNAbyblockingtypeIR-Msystems,whicharewidespreadinEubacteriaandArchaebacteria.
Applications
- IncreasethetransformationefficiencyofunmodifiedDNA2byblockingtypeIR-Msystems,whicharewidespreadinEubacteriaandArchaebacteria.3
- IncreasetheinsertionefficiencyofEZ-Tn5™Transposome™complexes.2
DNAtransformationcanbedifficulttoachieveinmanybacterialstrainsduetothepresenceofoneormorerestrictionandmodification(R-M)systemsthatcleaveunmodifiedDNAthatisrecognizedas"foreign."TypeOne™RestrictionInhibitor*providesapowerfulmethodforincreasingtransformationefficienciesinbacterialstrainswithtypeIR-Msystems.Developedasauniquepreparationofaphageproteincalledocr,1TypeOneInhibitorisreADIlyelectroporatedintocellsalongwithtransformingDNA.Invivo,theproteinactsasamoleculardecoythatblockstheDNA-bindingsiteoftypeIR-MenzymesandinhibitsDNAcleavage.
Benefits
- Easytouse:SimplyaddTypeOneRestrictionInhibitortounmodifiedDNAoraTransposomeandelectroporateusingstandardmethods.
- BlocksallknownfamiliesoftypeIR-Menzymes1andcanthereforebeusedtoincreasetransformationefficienciesinavarietyofbacterialcells.
Table1.EffectofTypeOne™RestrictionInhibitorontransformationefficiencies.
Strain(TypeIR-Msystem) | TypeOne™ Inhibitor | TypeofDNAorTransposome™ | Recombinants perµgDNA |
| S.typhimuriumLT2(StyLTIII) | pUC19(100pg) | 3.0×106 | |
| S.typhimuriumLT2(StyLTIII) | X | pUC19(100pg) | 3.0×108 |
| E.coliMG1655(EcoK1) | 48kbfosmid(50ng) | 3.0×103 | |
| E.coliMG1655(EcoK1) | X | 48kbfosmid(50ng) | 1.4×106 |
| S.typhimuriumLT2(StyLTIII) | EZ-Tn5™<R6Kγori/KAN-2>TnpTransposome™(1µl) | 1.3×104 | |
| S.typhimuriumLT2(StyLTIII) | X | EZ-Tn5™<R6Kγori/KAN-2>TnpTransposome™(1µl) | 1.0×106 |
References
- Walkinshaw,M.D.etal.(2002)Molec.Cell9,187.
- Hoffman,L.etal.(2002)EpicentreForum9(2),8.
- Murray,N.E.etal.(2000)Microbial.Molec.Biol.Rev.64,412.
*Coveredbyissuedand/orpendingpatents,exclusivelylicensedorassignedtoEpicentre®(anIllumina®Company).
美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。
厂家直采
正品保障
正规清关
及时交付
总部地址： 江苏省苏州市工业园区星湖街218号 苏州生物医药产业园一期（生物纳米园）A4-216 邮编：215125 工作时间：周一至周五9:00~18:00
技术支持： 
E-mail: info@ebiomall.com 短信: 18915418616
服务热线： 
4000-520-616(全国) 0512-67156496(苏州)
© 2015-2020 苏州蚂蚁淘生物科技有限公司 版权所有 中国
苏ICP备17049038号
苏公网安备 32059002001627号
友情链接：联系QQ:764052152
扫描二维码关注官方微信
获取最新促销及产品信息
