请使用支持JavaScript的浏览器! Lucigen-TransforMax™ EC100D™pir+andpir-116 ElectrocompetentE. coli-Lucigen/TransforMax™ EC100D™ pir+ Electrocompetent E.coli/ECP09500/5 x 100 µl
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公司介绍
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,...
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Lucigen/TransforMax™ EC100D™ pir+ Electrocompetent E.coli/ECP09500/5 x 100 µl

Size:5 x 100 µl
Lucigen/TransforMax™ EC100D™ pir+ Electrocompetent E.coli/ECP09500/5 x 100 µl
  • Lucigen/TransforMax™ EC100D™ pir+ Electrocompetent E.coli/ECP09500/5 x 100 µl
商品介绍

Rescue cloning of EZ-Tn5™ transposed elements

  • Rescue transposition mutants created using the EZ-Tn5™ R6KɣoriKan-2> Insertion Kit
    or Tnp Transposome™ Kit Formerly from Epicentre
  • Propagate plasmids tagged with the EZ-Tn5™ R6KɣoriKan-2> Insertion Kit

The TransforMax™ EC100D™ pir+ Electrocompetent E. coli and TransforMax™ EC100D™ pir-116 Electrocompetent E. coli each constitutively express the π protein (the pir gene product) for replication of plasmids containing the R6Kγ origin of replication (R6Kγori). The cells are derived from Epicentre's TransforMax™ EC100™ Electrocompetent E. coli by P1 phage transduction with a strain containing the pir+ or pir-116 gene linked to a dihydrofolate reductase (DHFR) Marker.

The TransforMax EC100D pir+ cells will maintain R6Kγori containing plasmids at approximately 15 copies per cell,1 for cloning of potentially toxic or unstable DNA sequences. The TransforMax EC100D pir-116 cells are for high-copy propagation of up to 250 rescue plasmid copies per cell.1

Benefits

  • Accepts large clones for unbiased propagation and stABIlity of large rescue clones.
  • lacZΔM15 for blue/white screening of recombinants.
  • Restriction minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] enables efficient cloning of methylated DNA.
  • Endonuclease minus (endA1) to ensure high yields of DNA.
  • Recombination minus (recA1) for greater stABIlity of large cloned inserts.

Genotypes

TransforMax EC100D pir+ Electrocompetent E. coli:
F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG pir+(DHFR)

TransforMax EC100D pir-116 Electrocompetent E. coli:
F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG pir-116(DHFR)

Transformation Efficiency
Greater than 5 × 109 colony forming units (cfu)/µg with pR6Kan control DNA.

References

  1. Metcalf, W.W. et al. (1994) Gene 138, 1.

ORDER INFORMATION

Each product contains 5 tubes each containing 100 µL of electrocompetent cells, which is enough cells for 10 electroporations. A control vector containing and R6Kγori is also included with each product.
品牌介绍

美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。

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