Clonerepetitivesequencesandlentivirallibraries

  • Stabilizedirectrepeatsandcreatelentiviralconstructs
  • GeneratelentiviralguideRNAlibraries
  • RecommendedinCRISPRGeCKOlibraryprotocols
  • Chooseelectrocompetentorchemicallycompetentcells
  • Highestefficiencycommerciallyavailablecellsforlentiviralcloning: over1×107cfu/µg(chem)or1×1010cfu/µg(electro)

TransformationofEnduraElectrocompetentCellswithGeCKOlentivirallibrariesmayrequireextraRecoveryMedium. OrderextraRecoveryMediumbelow.

Free Sample AvailableFreeSampleAvailable

Conserveclonesthatcontainunstablesequences!

MaintainunstableDNA.Lucigen’sEnduraCompetentCellsareacommonlyusedstrainforcloningsequencesthatsufferunwantedrecombinationeventsinotherstrains.Cloneswithinvertedrepeatsorothersequencespronetorecombinationarecommonlyfoundinretroviralgenes,andrequirecellssuchasEnduratobepropagatedstably.

Excellentvalueandefficiency!SwitchtoEnduracellstodayandexperienceahigherlevelofefficiencyandasavingsinyourcloningbudget.

ChemicalorElectrocompetent.Whichevermethodoftransformationyouprefer,Lucigencangiveyoubetterefficiencyand/orbetterprices.

Figure1.Costperreactionofchemicallycompetentcellsofclone-stabilizingstrainsfromothersuppliers.

Figure2.Transformationefficienciesofelectrocompetentclone-stabilizingstrains.

ORDERINFORMATION

Endura™CompetentCellsincludeControlDNAandRecoveryMedium,andarepackagedas SOLOs (1transformationpertube)orDUOs (2transformationspertube)asindicated.EnduraElectrocompetentcellsareprovidedinDUOsformatonly.RecoveryMediumisalsoavailableseparately.ThespecifiedtransformationefficienciesarewithpUCDNA,unlessindicatedotherwise.

PLEASENOTE: Bulkquantities(10timeslargerthanthelargestretailpackagesizebelow)and/orcustompackagingareavailableatveryattractivepricesforallLucigenCompetentCells. PleasecontactLucigen.

NOTE: TransformationofEnduraElectrocompetentCellswithGeCKOlentivirallibrariesmayrequireextraRecoveryMedium. TheZhanglabprotocol(availablefromAddgene)recommends~2mLofRecoveryMediumpertransformation. LucigenEndurakitsprovide1mLofRecoveryMediumpertransformation. OrderextraRecoveryMediumaboveorvisittheRecoveryMediumproductpagehere!