• Perfectforantibodyphagedisplayorpeptidephagedisplaylibrarycreation
  • ThehighestefficiencyTG1competentcellsavailable:≥4×1010cfu/µg.
  • YouronlysourceforelectrocompetentSS320andER2738cells
  • Ambersuppressorandnon-ambersuppressorlinesformaximumflexibility
  • HighefficiencyMC1061F-cellsforcloningorcontrolreactions
  • Createlargerlibraries;speeddiscovery
  • Bulkcustomdispensingavailablewithallofourphagedisplaycells

Free Sample AvailableFreeSampleAvailable

 

MC1061F-ElectrocompetentCells.Anon-ambersuppressorstrain,identicaltoSS320exceptthatitlackstheF′episome.Highlyefficient(≥4×1010cfu/µg)electrocompetentcellsforgeneralcloningorPhageDisplay.NotethatasanF-strain,itcannotbeusedforre-infectionbyfilamentousphage. Thisstrainnotincludedinthesamplekit.

  • TG1ElectrocompetentCells.Anambersuppressorstrain(supE)preparedashighlyefficient(≥4×1010cfu/µg)electrocompetentcellsforphagedisplaylibraryscreening.Thesecellsareusedforphagedisplayandproteinexpression.

  • SS320ElectrocompetentCells.Anon-ambersuppressorstrain(sometimescalledMC1061F')preparedashighlyefficient(≥4×1010cfu/µg)electrocompetentcellsforphagedisplaylibraryscreening.Thesecellshavethehighestavailabletransformationefficiencyofanystrainusedforphagedisplay.
  • ER2738ElectrocompetentCells.Anambersuppressorstrain(glnV)preparedashighlyefficient(≥2×1010cfu/µg)electrocompetentcellsforphagedisplaylibraryscreening.ThisstrainisrecommendedforusewithNewEnglandBiolab'sPh.D.™PhageDisplayKits.
Figure1.TransformationefficiencyofLucigen'selectrocompetentbacterialcellsforphagedisplaycomparedtocompetitor'sspecification.

Table1.CompetentCellsforPhageDisplaylibraries

    CustomCompetentCells

    BulkLucigencellsoryourstrainofE.coli,madetothehighestpossibletransfectionefficiencywithcustomdispensing!SeeCustomCompetentCellsfordetailedinformation.

    ORDERINFORMATION

    CompetentCellsincludeControlDNAandRecoveryMedium,andarepackagedasDUOs(2transformationspertube).RecoveryMediumisalsoavailableseparately.ThespecifiedtransformationefficienciesarewithpUCDNA,unlessindicatedotherwise.

    PLEASENOTE:Bulkquantities(10timeslargerthanthelargestretailpackagesizebelow)and/orcustompackagingareavailableatveryattractivepricesforallLucigenCompetentCells.PleasecontactLucigen.

    本文链接: http://lucigen.ebiomall.com/show-752310.html

    发布于 : 2019-05-01 阅读(0)
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    CellLines

    TransformationEfficiency
    (cfu/µgpUCDNA)

    CloningMethylatedDNA

    BAC,CosmidCloning

    Blue/WhiteScreening

    TG1Electrocompetent

    >4×1010

    YES

    NO

    YES
    IPTGinductionrequired

    SS320(MC1061F')Electrocompetent

    >4×1010

    YES

    NO

    YES
    IPTGinductionrequired

    MC1061F-Electrocompetent

    >4×1010

    YES

    NO

    YES
    IPTGinductionrequired

    ER2738Electrocompetent

    >2×1010

    YES

    NO

    YES
    IPTGinductionrequired