OptimizePCRreactionsthatusedifferentenzymesthantheFailSafe™System(e.g.Taq,Pfu,andTth).

  • Epicentre ProductFast,simpleoptimization
  • ExtremelyhighsensitivityandspecificityusingthePCREnhancerTechnology
  • MasterAmpPCRPreMixescontainallcomponentsneededforsuccessfulPCR
  • GeneratesPCRproductssuitableforbothTAcloningandblunt-endcloning
  • UsefortheoptimizationofPCRreactionsusingavarietyofdifferentenzymesincludingTaq,Pfu,Tth,Tflpolymerases
Applications
  • SimplifiedPCRoptimizationwithathermostableDNApolymeraseofchoice.
  • PCRofdifficultorhigh-GCtemplates.
  • PCRamplificationsupto20kb.
  • MultiplexPCR.

Forrapid,dependableoptimizationofPCR,weencourageyoutotrytheFailSafe™PCRSystemtoobtainthebestPCRresultswithanytemplateupto~20kb.However,ifyouprefertouseanotherenzyme,EpicentrealsohastwoMasterAmp™PCROptimizationKitsthatwillhelpyoutoobtainthebestpossiblePCRresultswiththermostableenzymesfrequentlyusedinPCR,suchasTaq,Pfu,Tth,TflDNApolymerases.TheseMasterAmpPCROptimizationKitscontainaseriesof12MasterAmpPCR2XPreMixesthathavebeenmeticulouslytestedandrepresentacompleterangeofPCRconditionsfrequentlyencountered.EachMasterAmp2XPreMixcontainsallcomponentsneededforPCRexceptfortheprimers,thetemplate,andthePCRenzyme.

First,usetheMasterAmp™PCROptimizationKit(Cat.No.MO7201)toperformPCRwithyourtemplate,primers,andeachofthe12MasterAmpPreMixes.SelectthePreMixthatgivesoptimalresults.Then,usethesameMasterAmpPCRPreMixtoobtainreliable,consistentPCRusngthesameDNAtarget/primerset.

STEP1.FindtheoptimalMasterAmpPCRPreMix.DoPCRwithyourtemplate,primersandPCRenzymeinthe12PreMixes.

A.A75%-GC-richApoEgeneregionwasamplifiedintotalhumangenomicDNAusingPreMixesA-L.MasterAmpPCRPreMixKproducedoptimalresultsinStep1.

STEP2.UsethesameMasterAmpPCRPreMixtoobtainreliable,consistentPCRofthesamesequence.

B.SubsequentPCRoftheApoEgenewiththesameprimerpairandMasterAmp™PreMixKgaveconsistentresults.SubsequentPCRresultsareconsistentandreliableusingtheoptimalPreMixinStep1.

MasterAmpPCROptimizationKits;MasterAmpTaqDNAPolymerase;MasterAmpTflDNAPolymerase.Purchaseofthisproductincludesanimmunityfromsuitunderpatentsspecifiedintheproductinserttouseonlytheamountpurchasedforthepurchaser'sowninternalresearch.Nootherpatentrights(suchas5´NucleaseProcesspatentrights)areconveyedexpressly,byimplication,orbyestoppel.FurtherinformationonpurchasinglicensesmaybeobtainedbycontactingtheDirectorofLicensing,AppliedBiosystems,850LincolnCentreDrive,FosterCity,California94404,USA.

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