• Cellsthatenablethemostchallenginglibraryconstructionandscreening.
  • Highestavailabletransformationefficienciesforlibraryconstructionandscreening.
  • OptimizedforhighyieldsofDNA.

LucigenofferstwobacterialstrainsdesignedforBAClibraryconstructionortransformationoflargeconstructs:

BAC-OptimizedReplicator™v2.0ElectrocompetentCells(>1×1010cfu/µgpKanRDNA). Thesecellsarerequiredforon-demand,copynumberamplificationofthepSMART®BACorpEZ™BACvectorsintheCopyRight®Kits,therebyincreasingDNAyields. BAC-OptimizedReplicatorCellsarecomponentsofLucigen’sCopyRight®CloningKits.

E.cloni10GBAC-OptimizedElectrocompetentCells(>1×107cfu/µg150kbBACDNA;>1×1010cfu/µgpUCDNA).ThefirstcompetentcellsdevelopedbyLucigenspecificallyforlargeDNAcloning. Manufacturedusinganovelprocedurethatmaximizestransformationefficiencieswithverylargeinserts,10GBAC-OptimizedCellsareanexcellentchoiceforBAClibraryconstructionandlargeconstructtransformation.

Table1.E.cloniCompetentCellsforBACorLargeFragmentCloning


E.cloniCellLines

TransformationEfficiency
(cfu/µgpUCorpKanRDNA)

CloningMethylatedDNA

BAC,CosmidCloning

Blue/WhiteScreening

10GBAC-OptimizedElectrocompetent

>1×1010

YES

YES

YES
withoutIPTGinduction

BAC-OptimizedReplicatorv2.0Electrocompetent

>1×1010

YES

YES

YES
IPTGinductionrequired

ORDERINFORMATION

CompetentCellsincludeControlDNAandRecoveryMedium,andarepackagedasDUOs(2transformationspertube)orFIVERs(5transformationspertube),asindicated.RecoveryMediumisalsoavailableseparately.ThespecifiedtransformationefficienciesarewithpUCDNA,unlessindicatedotherwise.

PLEASENOTE:Bulkquantities(10timeslargerthanthelargestretailpackagesizebelow)and/orcustompackagingareavailableatveryattractivepricesforallLucigenCompetentCells.PleasecontactLucigen.