• OutstandingCDNAsynthesis.

NxGen Enzymes IconM-MuLVReverseTranscriptaseisanRNA-dependentDNApolymerasewhichshowsnomeasurable3′→5′proofreADIngfunction.Thisenzymecancopyasingle-strandedDNAtemplateorperformcDNAsynthesisbyextendingaDNAprimerannealedtoanRNAtemplate.

Specifications

UnitDefinition:1unitisdefinedastheamountofenzymerequiredtoincorporate1nmolofdTTPintoacidinsolublematerialin10minutesat37°Cusingpolyr(A)/oligo(dT)asasubstrate.

Source:ArecombinantE.colistraincarryingtheMoloney-MurineLeukemiaVirusReverseTranscriptasegene.

M-MuLV Price Comparison
UnitConcentration200,000U/mL
Purity(SDS-PAGE)>99%
SSExonuclease2,000U<5.0% released
DSExonuclease2,000U<0.5% released
Endonuclease2,000U<10% converted
E.coli16SrDNAContamination2,000U<10 copies
Storage-20°C

ORDERINFORMATION

Suppliedat200,000U/mLin50mMTris-HCl,150mMNaCl,0.1mMEDTA,1mMdithiothreitol,0.1%NP-40Alternative,50%glycerol,pH7.6@25°C.Alsoprovidedis10XM-MuLVRTBufferwhichin1Xformiscomposedof50mMTris-HCl,75mMKCl,3mMMgCl2,10mMdithiothreitol,pH8.3@25°C.250,000Uissuppliedas5×50,000U.

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发布于 : 2019-06-26 阅读(0)
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