• Efficientlycloneanysizeinsertupto200kb.
  • CreatelibrariesfromA/T-orG/C-richgenomes.
  • Clonegeneclustersoroperons.
  • HighinsertstABIlity.
  • InducIBLecopynumber.

Lucigen'sCopyRight®v2.0BACcloningkitswithpSMART®BAC(Figure1)orpEZBAC(Figure2)vectorandBAC-OptimizedReplicator™v2.0ElectrocompetentCellsresultinunprecedentedaccuracy,reliability,andefficiencyinBACcloningandlibraryconstruction.ThesevectorsincorporatetheCloneSmart®transcription-freetechnology,whichminimizescloningbiasandenablescloningofotherwisetoxicgenes,alongwithinduciblecopynumberamplification(20-50X)forhighDNAyieldsandalacZ-sacBstufferfragmentthateliminatesuncutvectorfromrecombinants,resultinginzerobackground. UnlikeotherBACcloningsystems,Lucigen'sCloneSmarttechnologyassuresthateven"unclonable"insertsarestablymaintained.Sequencesarenotlostorrearrangedinthecloningprocess. CopyRightKitsoffersuperiorperformanceforBACcloningatamuchbetterpricethanotherkits. Figure3showsanexampleofaRandomShearBAClibrarygeneratedwithpSMARTBAC.

pSMART BAC Vector Map

pEZ BAC Vector Map

Figure1.CopyRightvectorpSMARTBAC. ori2,repE,IncC-single-copyoriginofreplication;oriV-inducibleoriginofreplication;parA,B,C-partitiongenes;Cm^r-chloramphenicol-resistancegene;cosN-lamBDapackagingsignal;T-CloneSmarttranscriptionterminators;sacB-sucrasegene.Approximatepositionsofsequencingprimersandtranscriptionterminatorsareindicated.

Figure2.CopyRightvectorpEZBAC.ori2,repE,IncC,single-copyoriginofreplication;oriV-inducibleoriginofreplication;parA,B,C,partitiongenes;Cm^r-chloramphenicol-resistancegene;cosN-lambdapackagingsignal;lacZ-alphapeptideofthebetagalactosidasegene.Approximatepositionsofsequencingprimersandtranscriptionterminatorsareindicated.

 

Arabidopsis BAC gel

Figure3.ArabidopsisRandomShearBACLibraryinthepSMARTBACvector.7,680cloneswereobtained,withanaverageinsertsizeof105kband~5Xgenomecoverage.

ConvenientSuccess
Efficientlyobtainyourcloneorcreateyourlibrary—theCopyRightv2.0CloningKitseliminatetediousvectorandcompetentcellpreparation,aswellastime-consumingQCexperiments. Kitsincludeoptimizedreagents,ligation-readyvector,highlyefficientelectrocompetentcells,detailedinstructions,andatrouble-shootingguide.

ORDERINFORMATION

EachCopyRight®v2.0BACCloningKitcontains:pre-cutvector(pSMARTBACwithachoiceofBamHIorHindIIIsites),CopyRightDNALigase,CopyRight5XLigationBuffer,PositiveControlInsertDNA,SequencingPrimers,BAC-OptimizedReplicatorv2.0ElectrocompetentCellsinDUOpackaging(2transformationspertube),PositiveControlInsertDNA,RecoveryMedium,ArabinoseInductionSolution,andcompleteprotocols.EachCopyRightpEZBACCloningKitcontains:pre-cutCopyRightvector(pEZBACBlunt),CloneSmartDNALigase,CloneDirect™10XLigationBuffer(includesATP),PositiveControlInsertDNA,ReplicatorInductionSolution,SequencingPrimers,andcompleteprotocols.KitswithcellsalsoincludeBAC-OptimizedReplicatorv2.0ElectrocompetentCells(>1×1010 cfu/µgDNA)packagedintwotransformationspertube(DUOs),PositiveControlPlasmid,andRecoveryMedium.

Pleasenotethatthefollowingvectorshavebeendiscontinued:pSMARTBACEcoRI(catalognumbers42031-1,42031-2)andpEZBACBamHI(catalognumbers42007-1,42007-2and42012-1).”